IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-45248-5.html
   My bibliography  Save this article

Phosphorylation of plasma membrane H+-ATPase Thr881 participates in light-induced stomatal opening

Author

Listed:
  • Yuki Hayashi

    (Nagoya University)

  • Kohei Fukatsu

    (Nagoya University)

  • Koji Takahashi

    (Nagoya University
    Nagoya University)

  • Satoru N. Kinoshita

    (Nagoya University)

  • Kyohei Kato

    (Nagoya University)

  • Taku Sakakibara

    (Nagoya University)

  • Keiko Kuwata

    (Nagoya University)

  • Toshinori Kinoshita

    (Nagoya University
    Nagoya University)

Abstract

Plasma membrane (PM) H+-ATPase is crucial for light-induced stomatal opening and phosphorylation of a penultimate residue, Thr948 (pen-Thr, numbering according to Arabidopsis AHA1) is required for enzyme activation. In this study, a comprehensive phosphoproteomic analysis using guard cell protoplasts from Vicia faba shows that both red and blue light increase the phosphorylation of Thr881, of PM H+-ATPase. Light-induced stomatal opening and the blue light-induced increase in stomatal conductance are reduced in transgenic Arabidopsis plants expressing mutant AHA1-T881A in aha1–9, whereas the blue light-induced phosphorylation of pen-Thr is unaffected. Auxin and photosynthetically active radiation induce the phosphorylation of both Thr881 and pen-Thr in etiolated seedlings and leaves, respectively. The dephosphorylation of phosphorylated Thr881 and pen-Thr are mediated by type 2 C protein phosphatase clade D isoforms. Taken together, Thr881 phosphorylation, in addition of the pen-Thr phosphorylation, are important for PM H+-ATPase function during physiological responses, such as light-induced stomatal opening in Arabidopsis thaliana.

Suggested Citation

  • Yuki Hayashi & Kohei Fukatsu & Koji Takahashi & Satoru N. Kinoshita & Kyohei Kato & Taku Sakakibara & Keiko Kuwata & Toshinori Kinoshita, 2024. "Phosphorylation of plasma membrane H+-ATPase Thr881 participates in light-induced stomatal opening," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45248-5
    DOI: 10.1038/s41467-024-45248-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-45248-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-45248-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Toshinori Kinoshita & Michio Doi & Noriyuki Suetsugu & Takatoshi Kagawa & Masamitsu Wada & Ken-ichiro Shimazaki, 2001. "phot1 and phot2 mediate blue light regulation of stomatal opening," Nature, Nature, vol. 414(6864), pages 656-660, December.
    2. Murukarthick Jayakodi & Agnieszka A. Golicz & Jonathan Kreplak & Lavinia I. Fechete & Deepti Angra & Petr Bednář & Elesandro Bornhofen & Hailin Zhang & Raphaël Boussageon & Sukhjiwan Kaur & Kwok Cheun, 2023. "The giant diploid faba genome unlocks variation in a global protein crop," Nature, Nature, vol. 615(7953), pages 652-659, March.
    3. Maoxing Zhang & Yin Wang & Xi Chen & Feiyun Xu & Ming Ding & Wenxiu Ye & Yuya Kawai & Yosuke Toda & Yuki Hayashi & Takamasa Suzuki & Houqing Zeng & Liang Xiao & Xin Xiao & Jin Xu & Shiwei Guo & Feng Y, 2021. "Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yusuke Aihara & Bumpei Maeda & Kanna Goto & Koji Takahashi & Mika Nomoto & Shigeo Toh & Wenxiu Ye & Yosuke Toda & Mami Uchida & Eri Asai & Yasuomi Tada & Kenichiro Itami & Ayato Sato & Kei Murakami & , 2023. "Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Ki-Hong Jung & Jinwon Lee & Chris Dardick & Young-Su Seo & Peijian Cao & Patrick Canlas & Jirapa Phetsom & Xia Xu & Shu Ouyang & Kyungsook An & Yun-Ja Cho & Geun-Cheol Lee & Yoosook Lee & Gynheung An , 2008. "Identification and Functional Analysis of Light-Responsive Unique Genes and Gene Family Members in Rice," PLOS Genetics, Public Library of Science, vol. 4(8), pages 1-19, August.
    3. Carmen Santos & Susana Trindade Leitão, 2023. "The Exceptionally Large Genomes of the Fabeae Tribe: Comparative Genomics and Applications in Abiotic and Biotic Stress Studies," Agriculture, MDPI, vol. 14(1), pages 1-21, December.
    4. Saashia Fuji & Shota Yamauchi & Naoyuki Sugiyama & Takayuki Kohchi & Ryuichi Nishihama & Ken-ichiro Shimazaki & Atsushi Takemiya, 2024. "Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H+-ATPase," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Fei Shen & Shixiao Xu & Qi Shen & Changwei Bi & Martin A. Lysak, 2023. "The allotetraploid horseradish genome provides insights into subgenome diversification and formation of critical traits," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    6. Niu, Yu & Suo, Yonglu & Niu, Xian, 2023. "Insights into the response mechanism of Fusarium sp. NF01 during lignite biodegradation using proteomic analysis," Energy, Elsevier, vol. 278(PB).
    7. Shey-Li Lim & Sabrina Flütsch & Jinhong Liu & Luca Distefano & Diana Santelia & Boon Leong Lim, 2022. "Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    8. Viktorija Vaštakaitė-Kairienė & Aušra Brazaitytė & Jurga Miliauskienė & Rūta Sutulienė & Kristina Laužikė & Akvilė Viršilė & Giedrė Samuolienė & Erik S. Runkle, 2022. "Photon Distribution of Sole-Source Lighting Affects the Mineral Nutrient Content of Microgreens," Agriculture, MDPI, vol. 12(8), pages 1-14, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45248-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.