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Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals

Author

Listed:
  • Yusuke Aihara

    (Nagoya University
    JST PRESTO)

  • Bumpei Maeda

    (Kwansei Gakuin University, Sanda)

  • Kanna Goto

    (Kwansei Gakuin University, Sanda)

  • Koji Takahashi

    (Nagoya University
    Nagoya University)

  • Mika Nomoto

    (JST PRESTO
    Nagoya University
    Nagoya University)

  • Shigeo Toh

    (Nagoya University
    Meijo University)

  • Wenxiu Ye

    (Nagoya University
    Shandong Laboratory of Advanced Agriculture Sciences in Weifang)

  • Yosuke Toda

    (Nagoya University
    Phytometrics Co., Ltd., Hamamatsu)

  • Mami Uchida

    (Nagoya University)

  • Eri Asai

    (Nagoya University)

  • Yasuomi Tada

    (Nagoya University
    Nagoya University)

  • Kenichiro Itami

    (Nagoya University
    Nagoya University)

  • Ayato Sato

    (Nagoya University)

  • Kei Murakami

    (Nagoya University
    JST PRESTO
    Kwansei Gakuin University, Sanda)

  • Toshinori Kinoshita

    (Nagoya University
    Nagoya University)

Abstract

Stomatal pores in the plant epidermis open and close to regulate gas exchange between leaves and the atmosphere. Upon light stimulation, the plasma membrane (PM) H+-ATPase is phosphorylated and activated via an intracellular signal transduction pathway in stomatal guard cells, providing a primary driving force for the opening movement. To uncover and manipulate this stomatal opening pathway, we screened a chemical library and identified benzyl isothiocyanate (BITC), a Brassicales-specific metabolite, as a potent stomatal-opening inhibitor that suppresses PM H+-ATPase phosphorylation. We further developed BITC derivatives with multiple isothiocyanate groups (multi-ITCs), which demonstrate inhibitory activity on stomatal opening up to 66 times stronger, as well as a longer duration of the effect and negligible toxicity. The multi-ITC treatment inhibits plant leaf wilting in both short (1.5 h) and long-term (24 h) periods. Our research elucidates the biological function of BITC and its use as an agrochemical that confers drought tolerance on plants by suppressing stomatal opening.

Suggested Citation

  • Yusuke Aihara & Bumpei Maeda & Kanna Goto & Koji Takahashi & Mika Nomoto & Shigeo Toh & Wenxiu Ye & Yosuke Toda & Mami Uchida & Eri Asai & Yasuomi Tada & Kenichiro Itami & Ayato Sato & Kei Murakami & , 2023. "Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38102-7
    DOI: 10.1038/s41467-023-38102-7
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    References listed on IDEAS

    as
    1. Maoxing Zhang & Yin Wang & Xi Chen & Feiyun Xu & Ming Ding & Wenxiu Ye & Yuya Kawai & Yosuke Toda & Yuki Hayashi & Takamasa Suzuki & Houqing Zeng & Liang Xiao & Xin Xiao & Jin Xu & Shiwei Guo & Feng Y, 2021. "Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Toshinori Kinoshita & Michio Doi & Noriyuki Suetsugu & Takatoshi Kagawa & Masamitsu Wada & Ken-ichiro Shimazaki, 2001. "phot1 and phot2 mediate blue light regulation of stomatal opening," Nature, Nature, vol. 414(6864), pages 656-660, December.
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    Cited by:

    1. Ayami Nakagawa & Krishna Mohan Sepuru & Shu Jan Yip & Hyemin Seo & Calvin M. Coffin & Kota Hashimoto & Zixuan Li & Yasutomo Segawa & Rie Iwasaki & Hiroe Kato & Daisuke Kurihara & Yusuke Aihara & Steph, 2024. "Chemical inhibition of stomatal differentiation by perturbation of the master-regulatory bHLH heterodimer via an ACT-Like domain," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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