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Cross-feeding promotes heterogeneity within yeast cell populations

Author

Listed:
  • Kevin K. Y. Hu

    (Monash University)

  • Ankita Suri

    (Monash University)

  • Geoff Dumsday

    (Commonwealth Scientific and Industrial Research Organisation)

  • Victoria S. Haritos

    (Monash University)

Abstract

Cellular heterogeneity in cell populations of isogenic origin is driven by intrinsic factors such as stochastic gene expression, as well as external factors like nutrient availability and interactions with neighbouring cells. Heterogeneity promotes population fitness and thus has important implications in antimicrobial and anticancer treatments, where stress tolerance plays a significant role. Here, we study plasmid retention dynamics within a population of plasmid-complemented ura3∆0 yeast cells, and show that the exchange of complementary metabolites between plasmid-carrying prototrophs and plasmid-free auxotrophs allows the latter to survive and proliferate in selective environments. This process also affects plasmid copy number in plasmid-carrying prototrophs, further promoting cellular functional heterogeneity. Finally, we show that targeted genetic engineering can be used to suppress cross-feeding and reduce the frequency of plasmid-free auxotrophs, or to exploit it for intentional population diversification and division of labour in co-culture systems.

Suggested Citation

  • Kevin K. Y. Hu & Ankita Suri & Geoff Dumsday & Victoria S. Haritos, 2024. "Cross-feeding promotes heterogeneity within yeast cell populations," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44623-y
    DOI: 10.1038/s41467-023-44623-y
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    References listed on IDEAS

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    1. Mikhail V. Zubkov, 2014. "Faster growth of the major prokaryotic versus eukaryotic CO2 fixers in the oligotrophic ocean," Nature Communications, Nature, vol. 5(1), pages 1-6, September.
    2. Marshall Louis Reaves & Brian D. Young & Aaron M. Hosios & Yi-Fan Xu & Joshua D. Rabinowitz, 2013. "Pyrimidine homeostasis is accomplished by directed overflow metabolism," Nature, Nature, vol. 500(7461), pages 237-241, August.
    3. Lucie Semenec & Amy K. Cain & Catherine J. Dawson & Qi Liu & Hue Dinh & Hannah Lott & Anahit Penesyan & Ram Maharjan & Francesca L. Short & Karl A. Hassan & Ian T. Paulsen, 2023. "Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    4. Chieh Hsu & Simone Scherrer & Antoine Buetti-Dinh & Prasuna Ratna & Julia Pizzolato & Vincent Jaquet & Attila Becskei, 2012. "Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution," Nature Communications, Nature, vol. 3(1), pages 1-10, January.
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