IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-023-44548-6.html
   My bibliography  Save this article

Hijacking of internal calcium dynamics by intracellularly residing viral rhodopsins

Author

Listed:
  • Ana-Sofia Eria-Oliveira

    (Univ. Grenoble Alpes, CEA, CNRS, IBS
    Université Côte d’Azur, CNRS, INSERM, iBV
    Ion Channel Science and Therapeutics
    Fédération Hospitalo-Universitaire InovPain, Cote d’Azur University, University Hospital Center Nice)

  • Mathilde Folacci

    (Univ. Grenoble Alpes, CEA, CNRS, IBS
    Ion Channel Science and Therapeutics
    Aarhus University)

  • Anne Amandine Chassot

    (Université Côte d’Azur, CNRS, INSERM, iBV
    Ion Channel Science and Therapeutics
    Fédération Hospitalo-Universitaire InovPain, Cote d’Azur University, University Hospital Center Nice)

  • Sandrine Fedou

    (Univ. Bordeaux, Inserm, BRIC, UMR)

  • Nadine Thézé

    (Univ. Bordeaux, Inserm, BRIC, UMR)

  • Dmitrii Zabelskii

    (European XFEL)

  • Alexey Alekseev

    (Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen
    Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen)

  • Ernst Bamberg

    (Max Planck Institute of Biophysics)

  • Valentin Gordeliy

    (Univ. Grenoble Alpes, CEA, CNRS, IBS
    Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich)

  • Guillaume Sandoz

    (Université Côte d’Azur, CNRS, INSERM, iBV
    Ion Channel Science and Therapeutics
    Fédération Hospitalo-Universitaire InovPain, Cote d’Azur University, University Hospital Center Nice)

  • Michel Vivaudou

    (Univ. Grenoble Alpes, CEA, CNRS, IBS
    Ion Channel Science and Therapeutics)

Abstract

Rhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they are also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. Here, we examine the properties of OLPVR1 (Organic Lake Phycodnavirus Rhodopsin) and two other type 1 viral channelrhodopsins (VCR1s), and demonstrate that VCR1s accumulate exclusively intracellularly, and, upon illumination, induce calcium release from intracellular IP3-dependent stores. In vivo, this light-induced calcium release is sufficient to remote control muscle contraction in VCR1-expressing tadpoles. VCR1s natively confer light-induced Ca2+ release, suggesting a distinct mechanism for reshaping the response to light of virus-infected algae. The ability of VCR1s to photorelease calcium without altering plasma membrane electrical properties marks them as potential precursors for optogenetics tools, with potential applications in basic research and medicine.

Suggested Citation

  • Ana-Sofia Eria-Oliveira & Mathilde Folacci & Anne Amandine Chassot & Sandrine Fedou & Nadine Thézé & Dmitrii Zabelskii & Alexey Alekseev & Ernst Bamberg & Valentin Gordeliy & Guillaume Sandoz & Michel, 2024. "Hijacking of internal calcium dynamics by intracellularly residing viral rhodopsins," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44548-6
    DOI: 10.1038/s41467-023-44548-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-44548-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-44548-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Dmitry Bratanov & Kirill Kovalev & Jan-Philipp Machtens & Roman Astashkin & Igor Chizhov & Dmytro Soloviov & Dmytro Volkov & Vitaly Polovinkin & Dmitrii Zabelskii & Thomas Mager & Ivan Gushchin & Taty, 2019. "Unique structure and function of viral rhodopsins," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    2. Mohammad Moniruzzaman & Carolina A. Martinez-Gutierrez & Alaina R. Weinheimer & Frank O. Aylward, 2020. "Dynamic genome evolution and complex virocell metabolism of globally-distributed giant viruses," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    3. Dmitrii Zabelskii & Alexey Alekseev & Kirill Kovalev & Vladan Rankovic & Taras Balandin & Dmytro Soloviov & Dmitry Bratanov & Ekaterina Savelyeva & Elizaveta Podolyak & Dmytro Volkov & Svetlana Vagano, 2020. "Viral rhodopsins 1 are an unique family of light-gated cation channels," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Alexandra Bessenay & Hugo Bisio & Lucid Belmudes & Yohann Couté & Lionel Bertaux & Jean-Michel Claverie & Chantal Abergel & Sandra Jeudy & Matthieu Legendre, 2024. "Complex transcriptional regulations of a hyperparasitic quadripartite system in giant viruses infecting protists," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Hugo Bisio & Matthieu Legendre & Claire Giry & Nadege Philippe & Jean-Marie Alempic & Sandra Jeudy & Chantal Abergel, 2023. "Evolution of giant pandoravirus revealed by CRISPR/Cas9," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Sofia Rigou & Sébastien Santini & Chantal Abergel & Jean-Michel Claverie & Matthieu Legendre, 2022. "Past and present giant viruses diversity explored through permafrost metagenomics," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Patrick Arthofer & Florian Panhölzl & Vincent Delafont & Alban Hay & Siegfried Reipert & Norbert Cyran & Stefanie Wienkoop & Anouk Willemsen & Ines Sifaoui & Iñigo Arberas-Jiménez & Frederik Schulz & , 2024. "A giant virus infecting the amoeboflagellate Naegleria," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Lingjie Meng & Tom O. Delmont & Morgan Gaïa & Eric Pelletier & Antonio Fernàndez-Guerra & Samuel Chaffron & Russell Y. Neches & Junyi Wu & Hiroto Kaneko & Hisashi Endo & Hiroyuki Ogata, 2023. "Genomic adaptation of giant viruses in polar oceans," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    6. Qianqian Shao & Irina V. Agarkova & Eric A. Noel & David D. Dunigan & Yunshu Liu & Aohan Wang & Mingcheng Guo & Linlin Xie & Xinyue Zhao & Michael G. Rossmann & James L. Etten & Thomas Klose & Qiangli, 2022. "Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44548-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.