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STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation

Author

Listed:
  • Yu Chen

    (Nanjing Medical University
    Nanjing Medical University)

  • Mengjiao Luo

    (Nanjing Medical University)

  • Haixia Tu

    (Nanjing Medical University
    Sir Run Run Hospital, Nanjing Medical University)

  • Yaling Qi

    (Nanjing Medical University)

  • Yueshuai Guo

    (Nanjing Medical University)

  • Xiangzheng Zhang

    (Nanjing Medical University)

  • Yiqiang Cui

    (Nanjing Medical University)

  • Mengmeng Gao

    (Nanjing Medical University)

  • Xin Zhou

    (Nanjing Medical University)

  • Tianyu Zhu

    (Nanjing Medical University)

  • Hui Zhu

    (Nanjing Medical University)

  • Chenghao Situ

    (Nanjing Medical University)

  • Yan Li

    (Sir Run Run Hospital, Nanjing Medical University)

  • Xuejiang Guo

    (Nanjing Medical University)

Abstract

Tubulin-based microtubule is a core component of flagella axoneme and essential for sperm motility and male fertility. Structural components of the axoneme have been well explored. However, how tubulin folding is regulated in sperm flagella formation is still largely unknown. Here, we report a germ cell-specific co-factor of CCT complex, STYXL1. Deletion of Styxl1 results in male infertility and microtubule defects of sperm flagella. Proteomic analysis of Styxl1-/- sperm reveals abnormal downregulation of flagella-related proteins including tubulins. The N-terminal rhodanese-like domain of STYXL1 is important for its interactions with CCT complex subunits, CCT1, CCT6 and CCT7. Styxl1 deletion leads to defects in CCT complex assembly and tubulin polymerization. Collectively, our findings reveal the vital roles of germ cell-specific STYXL1 in CCT-facilitated tubulin folding and sperm flagella development.

Suggested Citation

  • Yu Chen & Mengjiao Luo & Haixia Tu & Yaling Qi & Yueshuai Guo & Xiangzheng Zhang & Yiqiang Cui & Mengmeng Gao & Xin Zhou & Tianyu Zhu & Hui Zhu & Chenghao Situ & Yan Li & Xuejiang Guo, 2024. "STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44337-1
    DOI: 10.1038/s41467-023-44337-1
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    1. Yin Shen & Feng Yue & David F. McCleary & Zhen Ye & Lee Edsall & Samantha Kuan & Ulrich Wagner & Jesse Dixon & Leonard Lee & Victor V. Lobanenkov & Bing Ren, 2012. "A map of the cis-regulatory sequences in the mouse genome," Nature, Nature, vol. 488(7409), pages 116-120, August.
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