IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-43589-1.html
   My bibliography  Save this article

Interrogating ligand-receptor interactions using highly sensitive cellular biosensors

Author

Listed:
  • Maximilian A. Funk

    (Medical University of Vienna)

  • Judith Leitner

    (Medical University of Vienna)

  • Marlene C. Gerner

    (University of Applied Sciences FH Campus Wien)

  • Jasmin M. Hammerler

    (University of Applied Sciences FH Campus Wien)

  • Benjamin Salzer

    (St. Anna Children’s Cancer Research Institute
    Christian Doppler Laboratory for Next Generation CAR T Cells)

  • Manfred Lehner

    (St. Anna Children’s Cancer Research Institute
    Christian Doppler Laboratory for Next Generation CAR T Cells)

  • Claire Battin

    (Medical University of Vienna)

  • Simon Gumpelmair

    (Medical University of Vienna)

  • Karin Stiasny

    (Medical University of Vienna)

  • Katharina Grabmeier-Pfistershammer

    (Medical University of Vienna)

  • Peter Steinberger

    (Medical University of Vienna)

Abstract

Interactions of membrane-resident proteins are important targets for therapeutic interventions but most methods to study them are either costly, laborious or fail to reflect the physiologic interaction of membrane resident proteins in trans. Here we describe highly sensitive cellular biosensors as a tool to study receptor-ligand pairs. They consist of fluorescent reporter cells that express chimeric receptors harboring ectodomains of cell surface molecules and intracellular signaling domains. We show that a broad range of molecules can be integrated into this platform and we demonstrate its applicability to highly relevant research areas, including the characterization of immune checkpoints and the probing of cells for the presence of receptors or ligands. The platform is suitable to evaluate the interactions of viral proteins with host receptors and to test for neutralization capability of drugs or biological samples. Our results indicate that cellular biosensors have broad utility as a tool to study protein-interactions.

Suggested Citation

  • Maximilian A. Funk & Judith Leitner & Marlene C. Gerner & Jasmin M. Hammerler & Benjamin Salzer & Manfred Lehner & Claire Battin & Simon Gumpelmair & Karin Stiasny & Katharina Grabmeier-Pfistershammer, 2023. "Interrogating ligand-receptor interactions using highly sensitive cellular biosensors," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43589-1
    DOI: 10.1038/s41467-023-43589-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-43589-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-43589-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Peng Zhou & Xing-Lou Yang & Xian-Guang Wang & Ben Hu & Lei Zhang & Wei Zhang & Hao-Rui Si & Yan Zhu & Bei Li & Chao-Lin Huang & Hui-Dong Chen & Jing Chen & Yun Luo & Hua Guo & Ren-Di Jiang & Mei-Qin L, 2020. "Addendum: A pneumonia outbreak associated with a new coronavirus of probable bat origin," Nature, Nature, vol. 588(7836), pages 6-6, December.
    2. S. Michael Chin & Christopher R. Kimberlin & Zygy Roe-Zurz & Pamela Zhang & Allison Xu & Sindy Liao-Chan & Debasish Sen & Andrew R. Nager & Nicole Schirle Oakdale & Colleen Brown & Feng Wang & Yuting , 2018. "Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    3. Peng Zhou & Xing-Lou Yang & Xian-Guang Wang & Ben Hu & Lei Zhang & Wei Zhang & Hao-Rui Si & Yan Zhu & Bei Li & Chao-Lin Huang & Hui-Dong Chen & Jing Chen & Yun Luo & Hua Guo & Ren-Di Jiang & Mei-Qin L, 2020. "A pneumonia outbreak associated with a new coronavirus of probable bat origin," Nature, Nature, vol. 579(7798), pages 270-273, March.
    4. Elisabetta Cameroni & John E. Bowen & Laura E. Rosen & Christian Saliba & Samantha K. Zepeda & Katja Culap & Dora Pinto & Laura A. VanBlargan & Anna Marco & Julia Iulio & Fabrizia Zatta & Hannah Kaise, 2022. "Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift," Nature, Nature, vol. 602(7898), pages 664-670, February.
    5. Jian Shang & Gang Ye & Ke Shi & Yushun Wan & Chuming Luo & Hideki Aihara & Qibin Geng & Ashley Auerbach & Fang Li, 2020. "Structural basis of receptor recognition by SARS-CoV-2," Nature, Nature, vol. 581(7807), pages 221-224, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Peter Radvak & Hyung-Joon Kwon & Martina Kosikova & Uriel Ortega-Rodriguez & Ruoxuan Xiang & Je-Nie Phue & Rong-Fong Shen & James Rozzelle & Neeraj Kapoor & Taylor Rabara & Jeff Fairman & Hang Xie, 2021. "SARS-CoV-2 B.1.1.7 (alpha) and B.1.351 (beta) variants induce pathogenic patterns in K18-hACE2 transgenic mice distinct from early strains," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    2. Hossein Hozhabri & Francesca Piceci Sparascio & Hamidreza Sohrabi & Leila Mousavifar & René Roy & Daniela Scribano & Alessandro De Luca & Cecilia Ambrosi & Meysam Sarshar, 2020. "The Global Emergency of Novel Coronavirus (SARS-CoV-2): An Update of the Current Status and Forecasting," IJERPH, MDPI, vol. 17(16), pages 1-35, August.
    3. Fabian Zech & Daniel Schniertshauer & Christoph Jung & Alexandra Herrmann & Arne Cordsmeier & Qinya Xie & Rayhane Nchioua & Caterina Prelli Bozzo & Meta Volcic & Lennart Koepke & Janis A. Müller & Jan, 2021. "Spike residue 403 affects binding of coronavirus spikes to human ACE2," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    4. Gang Ye & Bin Liu & Fang Li, 2022. "Cryo-EM structure of a SARS-CoV-2 omicron spike protein ectodomain," Nature Communications, Nature, vol. 13(1), pages 1-7, December.
    5. Tomokazu Yamaguchi & Midori Hoshizaki & Takafumi Minato & Satoru Nirasawa & Masamitsu N. Asaka & Mayumi Niiyama & Masaki Imai & Akihiko Uda & Jasper Fuk-Woo Chan & Saori Takahashi & Jianbo An & Akari , 2021. "ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    6. Zepeng Xu & Xinrui Kang & Pu Han & Pei Du & Linjie Li & Anqi Zheng & Chuxia Deng & Jianxun Qi & Xin Zhao & Qihui Wang & Kefang Liu & George Fu Gao, 2022. "Binding and structural basis of equine ACE2 to RBDs from SARS-CoV, SARS-CoV-2 and related coronaviruses," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    7. Jun-Yu Si & Yuan-Mei Chen & Ye-Hui Sun & Meng-Xue Gu & Mei-Ling Huang & Lu-Lu Shi & Xiao Yu & Xiao Yang & Qing Xiong & Cheng-Bao Ma & Peng Liu & Zheng-Li Shi & Huan Yan, 2024. "Sarbecovirus RBD indels and specific residues dictating multi-species ACE2 adaptiveness," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    8. Shahadat Uddin & Arif Khan & Haohui Lu & Fangyu Zhou & Shakir Karim, 2022. "Suburban Road Networks to Explore COVID-19 Vulnerability and Severity," IJERPH, MDPI, vol. 19(4), pages 1-9, February.
    9. Kirsten R.C. Hensgens & Inge H.T. van Rensen & Anita W. Lekx & Frits H.M. van Osch & Lieve H.H. Knarren & Caroline E. Wyers & Joop P. van den Bergh & Dennis G. Barten, 2021. "Sort and Sieve: Pre-Triage Screening of Patients with Suspected COVID-19 in the Emergency Department," IJERPH, MDPI, vol. 18(17), pages 1-11, September.
    10. Quan-Hoang Vuong & Tam-Tri Le & Viet-Phuong La & Huyen Thanh Thanh Nguyen & Manh-Toan Ho & Quy Khuc & Minh-Hoang Nguyen, 2022. "Covid-19 vaccines production and societal immunization under the serendipity-mindsponge-3D knowledge management theory and conceptual framework," Palgrave Communications, Palgrave Macmillan, vol. 9(1), pages 1-12, December.
    11. Hengrui Liu & Sho Iketani & Arie Zask & Nisha Khanizeman & Eva Bednarova & Farhad Forouhar & Brandon Fowler & Seo Jung Hong & Hiroshi Mohri & Manoj S. Nair & Yaoxing Huang & Nicholas E. S. Tay & Sumin, 2022. "Development of optimized drug-like small molecule inhibitors of the SARS-CoV-2 3CL protease for treatment of COVID-19," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    12. Graziella Orrù & Ciro Conversano & Eleonora Malloggi & Francesca Francesconi & Rebecca Ciacchini & Angelo Gemignani, 2020. "Neurological Complications of COVID-19 and Possible Neuroinvasion Pathways: A Systematic Review," IJERPH, MDPI, vol. 17(18), pages 1-18, September.
    13. Gleidson Sobreira Leite & Adriano Bessa Albuquerque & Plácido Rogerio Pinheiro, 2021. "Applications of Technological Solutions in Primary Ways of Preventing Transmission of Respiratory Infectious Diseases—A Systematic Literature Review," IJERPH, MDPI, vol. 18(20), pages 1-50, October.
    14. Britton Boras & Rhys M. Jones & Brandon J. Anson & Dan Arenson & Lisa Aschenbrenner & Malina A. Bakowski & Nathan Beutler & Joseph Binder & Emily Chen & Heather Eng & Holly Hammond & Jennifer Hammond , 2021. "Preclinical characterization of an intravenous coronavirus 3CL protease inhibitor for the potential treatment of COVID19," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    15. Susanne Kessler & Bradly Burke & Geoffroy Andrieux & Jan Schinköthe & Lea Hamberger & Johannes Kacza & Shijun Zhan & Clara Reasoner & Taru S. Dutt & Maria Kaukab Osman & Marcela Henao-Tamayo & Julian , 2024. "Deciphering bat influenza H18N11 infection dynamics in male Jamaican fruit bats on a single-cell level," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    16. Yongzhu Xiong & Yunpeng Wang & Feng Chen & Mingyong Zhu, 2020. "Spatial Statistics and Influencing Factors of the COVID-19 Epidemic at Both Prefecture and County Levels in Hubei Province, China," IJERPH, MDPI, vol. 17(11), pages 1-26, May.
    17. Eugene Song & Jae-Eun Lee & Seola Kwon, 2021. "Effect of Public Empathy with Infection-Control Guidelines on Infection-Prevention Attitudes and Behaviors: Based on the Case of COVID-19," IJERPH, MDPI, vol. 18(24), pages 1-18, December.
    18. Fabiana Fiasca & Mauro Minelli & Dominga Maio & Martina Minelli & Ilaria Vergallo & Stefano Necozione & Antonella Mattei, 2020. "Associations between COVID-19 Incidence Rates and the Exposure to PM2.5 and NO 2 : A Nationwide Observational Study in Italy," IJERPH, MDPI, vol. 17(24), pages 1-10, December.
    19. Małgorzata Dudzińska & Marta Gwiaździńska-Goraj & Aleksandra Jezierska-Thöle, 2022. "Social Factors as Major Determinants of Rural Development Variation for Predicting Epidemic Vulnerability: A Lesson for the Future," IJERPH, MDPI, vol. 19(21), pages 1-24, October.
    20. James, Nick & Menzies, Max, 2023. "Collective infectivity of the pandemic over time and association with vaccine coverage and economic development," Chaos, Solitons & Fractals, Elsevier, vol. 176(C).

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43589-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.