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Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors

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  • Xiaosi Wang

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Hubei Hongshan Laboratory)

  • Junwen Zhu

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Houpeng Wang

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    Hubei Hongshan Laboratory)

  • Wenqi Deng

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Shengbo Jiao

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yaqing Wang

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Hubei Hongshan Laboratory)

  • Mudan He

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Hubei Hongshan Laboratory)

  • Fenghua Zhang

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Tao Liu

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yongkang Hao

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Ding Ye

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Hubei Hongshan Laboratory)

  • Yonghua Sun

    (State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Hubei Hongshan Laboratory)

Abstract

The combination of genome editing and primordial germ cell (PGC) transplantation has enormous significance in the study of developmental biology and genetic breeding, despite its low efficiency due to limited number of donor PGCs. Here, we employ a combination of germplasm factors to convert blastoderm cells into induced PGCs (iPGCs) in zebrafish and obtain functional gametes either through iPGC transplantation or via the single blastomere overexpression of germplasm factors. Zebrafish-derived germplasm factors convert blastula cells of Gobiocypris rarus into iPGCs, and Gobiocypris rarus spermatozoa can be produced by iPGC-transplanted zebrafish. Moreover, the combination of genome knock-in and iPGC transplantation perfectly resolves the contradiction between high knock-in efficiency and early lethality during embryonic stages and greatly improves the efficiency of genome knock-in. Together, we present an efficient method for generating PGCs in a teleost, a technique that will have a strong impact in basic research and aquaculture.

Suggested Citation

  • Xiaosi Wang & Junwen Zhu & Houpeng Wang & Wenqi Deng & Shengbo Jiao & Yaqing Wang & Mudan He & Fenghua Zhang & Tao Liu & Yongkang Hao & Ding Ye & Yonghua Sun, 2023. "Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43587-3
    DOI: 10.1038/s41467-023-43587-3
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    References listed on IDEAS

    as
    1. Yutaka Takeuchi & Goro Yoshizaki & Toshio Takeuchi, 2004. "Surrogate broodstock produces salmonids," Nature, Nature, vol. 430(7000), pages 629-630, August.
    2. Kazuhiro Murakami & Ufuk Günesdogan & Jan J. Zylicz & Walfred W. C. Tang & Roopsha Sengupta & Toshihiro Kobayashi & Shinseog Kim & Richard Butler & Sabine Dietmann & M. Azim Surani, 2016. "NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers," Nature, Nature, vol. 529(7586), pages 403-407, January.
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