IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-43495-6.html
   My bibliography  Save this article

A dual role of RBM42 in modulating splicing and translation of CDKN1A/p21 during DNA damage response

Author

Listed:
  • Bella M. Ben-Oz

    (Technion - Israel Institute of Technology)

  • Feras E. Machour

    (Technion - Israel Institute of Technology)

  • Marian Nicola

    (Technion - Israel Institute of Technology)

  • Amir Argoetti

    (Technion - Israel Institute of Technology)

  • Galia Polyak

    (Technion - Israel Institute of Technology)

  • Rawad Hanna

    (Technion - Israel Institute of Technology)

  • Oded Kleifeld

    (Technion - Israel Institute of Technology)

  • Yael Mandel-Gutfreund

    (Technion - Israel Institute of Technology)

  • Nabieh Ayoub

    (Technion - Israel Institute of Technology)

Abstract

p53-mediated cell cycle arrest during DNA damage is dependent on the induction of p21 protein, encoded by the CDKN1A gene. p21 inhibits cyclin-dependent kinases required for cell cycle progression to guarantee accurate repair of DNA lesions. Hence, fine-tuning of p21 levels is crucial to preserve genomic stability. Currently, the multilayered regulation of p21 levels during DNA damage is not fully understood. Herein, we identify the human RNA binding motif protein 42 (RBM42) as a regulator of p21 levels during DNA damage. Genome-wide transcriptome and interactome analysis reveals that RBM42 alters the expression of p53-regulated genes during DNA damage. Specifically, we demonstrate that RBM42 facilitates CDKN1A splicing by counteracting the splicing inhibitory effect of RBM4 protein. Unexpectedly, we also show that RBM42, underpins translation of various splicing targets, including CDKN1A. Concordantly, transcriptome-wide mapping of RBM42-RNA interactions using eCLIP further substantiates the dual function of RBM42 in regulating splicing and translation of its target genes, including CDKN1A. Collectively, our data show that RBM42 couples splicing and translation machineries to fine-tune gene expression during DNA damage response.

Suggested Citation

  • Bella M. Ben-Oz & Feras E. Machour & Marian Nicola & Amir Argoetti & Galia Polyak & Rawad Hanna & Oded Kleifeld & Yael Mandel-Gutfreund & Nabieh Ayoub, 2023. "A dual role of RBM42 in modulating splicing and translation of CDKN1A/p21 during DNA damage response," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43495-6
    DOI: 10.1038/s41467-023-43495-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-43495-6
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-43495-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Carsten Boesler & Norbert Rigo & Maria M. Anokhina & Marcel J. Tauchert & Dmitry E. Agafonov & Berthold Kastner & Henning Urlaub & Ralf Ficner & Cindy L. Will & Reinhard Lührmann, 2016. "A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity," Nature Communications, Nature, vol. 7(1), pages 1-12, November.
    2. Marina Farkas & Hideharu Hashimoto & Yingtao Bi & Ramana V. Davuluri & Lois Resnick-Silverman & James J. Manfredi & Erik W. Debler & Steven B. McMahon, 2021. "Distinct mechanisms control genome recognition by p53 at its target genes linked to different cell fates," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    3. James Uniacke & Chet E. Holterman & Gabriel Lachance & Aleksandra Franovic & Mathieu D. Jacob & Marc R. Fabian & Josianne Payette & Martin Holcik & Arnim Pause & Stephen Lee, 2012. "An oxygen-regulated switch in the protein synthesis machinery," Nature, Nature, vol. 486(7401), pages 126-129, June.
    4. Tom Maniatis & Robin Reed, 2002. "An extensive network of coupling among gene expression machines," Nature, Nature, vol. 416(6880), pages 499-506, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xiechao Zhan & Yichen Lu & Yigong Shi, 2024. "Molecular basis for the activation of human spliceosome," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    2. Mykola Roiuk & Marilena Neff & Aurelio A. Teleman, 2024. "eIF4E-independent translation is largely eIF3d-dependent," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    3. Lucia Coscujuela Tarrero & Valeria Famà & Giacomo D’Andrea & Simone Maestri & Anna Polo & Stefano Biffo & Mattia Furlan & Mattia Pelizzola, 2024. "Nanodynamo quantifies subcellular RNA dynamics revealing extensive coupling between steps of the RNA life cycle," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43495-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.