IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-43437-2.html
   My bibliography  Save this article

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms

Author

Listed:
  • Christian Reuter

    (Biocenter, Julius-Maximilians-Universitaet of Wuerzburg
    University Hospital Wuerzburg)

  • Laura Hauf

    (Biocenter, Julius-Maximilians-Universitaet of Wuerzburg)

  • Fabian Imdahl

    (Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Center for Infection Research (HZI)
    Julius-Maximilians-Universitaet of Wuerzburg)

  • Rituparno Sen

    (Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Center for Infection Research (HZI))

  • Ehsan Vafadarnejad

    (Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Center for Infection Research (HZI))

  • Philipp Fey

    (Translational Center Regenerative Therapies, Fraunhofer ISC)

  • Tamara Finger

    (Translational Center Regenerative Therapies, Fraunhofer ISC)

  • Nicola G. Jones

    (Biocenter, Julius-Maximilians-Universitaet of Wuerzburg)

  • Heike Walles

    (Translational Center Regenerative Therapies, Fraunhofer ISC
    Otto-von-Guericke University)

  • Lars Barquist

    (Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Center for Infection Research (HZI))

  • Antoine-Emmanuel Saliba

    (Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Center for Infection Research (HZI)
    Julius-Maximilians-Universitaet of Wuerzburg)

  • Florian Groeber-Becker

    (University Hospital Wuerzburg
    Translational Center Regenerative Therapies, Fraunhofer ISC)

  • Markus Engstler

    (Biocenter, Julius-Maximilians-Universitaet of Wuerzburg)

Abstract

Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle-arrested metacyclic life cycle stage into mammalian skin at the site of the fly’s bite. We introduce an advanced human skin equivalent and use tsetse flies to naturally infect the skin with trypanosomes. We detail the chronological order of the parasites’ development in the skin by single-cell RNA sequencing and find a rapid activation of metacyclic trypanosomes and differentiation to proliferative parasites. Here we show that after the establishment of a proliferative population, the parasites enter a reversible quiescent state characterized by slow replication and a strongly reduced metabolism. We term these quiescent trypanosomes skin tissue forms, a parasite population that may play an important role in maintaining the infection over long time periods and in asymptomatic infected individuals.

Suggested Citation

  • Christian Reuter & Laura Hauf & Fabian Imdahl & Rituparno Sen & Ehsan Vafadarnejad & Philipp Fey & Tamara Finger & Nicola G. Jones & Heike Walles & Lars Barquist & Antoine-Emmanuel Saliba & Florian Gr, 2023. "Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43437-2
    DOI: 10.1038/s41467-023-43437-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-43437-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-43437-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sandra Trindade & Mariana Niz & Mariana Costa-Sequeira & Tiago Bizarra-Rebelo & Fábio Bento & Mario Dejung & Marta Valido Narciso & Lara López-Escobar & João Ferreira & Falk Butter & Frédéric Bringaud, 2022. "Slow growing behavior in African trypanosomes during adipose tissue colonization," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Samuel Dean & Rosa Marchetti & Kiaran Kirk & Keith R. Matthews, 2009. "A surface transporter family conveys the trypanosome differentiation signal," Nature, Nature, vol. 459(7244), pages 213-217, May.
    3. Shuxiong Wang & Michael L. Drummond & Christian F. Guerrero-Juarez & Eric Tarapore & Adam L. MacLean & Adam R. Stabell & Stephanie C. Wu & Guadalupe Gutierrez & Bao T. That & Claudia A. Benavente & Qi, 2020. "Single cell transcriptomics of human epidermis identifies basal stem cell transition states," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jiwoon Park & Eliah G. Overbey & S. Anand Narayanan & JangKeun Kim & Braden T. Tierney & Namita Damle & Deena Najjar & Krista A. Ryon & Jacqueline Proszynski & Ashley Kleinman & Jeremy Wain Hirschberg, 2024. "Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Sarah M. Lloyd & Daniel B. Leon & Mari O. Brady & Deborah Rodriguez & Madison P. McReynolds & Junghun Kweon & Amy E. Neely & Laura A. Blumensaadt & Patric J. Ho & Xiaomin Bao, 2022. "CDK9 activity switch associated with AFF1 and HEXIM1 controls differentiation initiation from epidermal progenitors," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    3. Daniel Haensel & Sadhana Gaddam & Nancy Y. Li & Fernanda Gonzalez & Tiffany Patel & Jeffrey M. Cloutier & Kavita Y. Sarin & Jean Y. Tang & Kerri E. Rieger & Sumaira Z. Aasi & Anthony E. Oro, 2022. "LY6D marks pre-existing resistant basosquamous tumor subpopulations," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Juan F. Quintana & Praveena Chandrasegaran & Matthew C. Sinton & Emma M. Briggs & Thomas D. Otto & Rhiannon Heslop & Calum Bentley-Abbot & Colin Loney & Luis de Lecea & Neil A. Mabbott & Annette MacLe, 2022. "Single cell and spatial transcriptomic analyses reveal microglia-plasma cell crosstalk in the brain during Trypanosoma brucei infection," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    5. Mabel Deladem Tettey & Federico Rojas & Keith R. Matthews, 2022. "Extracellular release of two peptidases dominates generation of the trypanosome quorum-sensing signal," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    6. Mathieu Cayla & Christos Spanos & Kirsty McWilliam & Eliza Waskett & Juri Rappsilber & Keith R. Matthews, 2024. "Differentiation granules, a dynamic regulator of T. brucei development," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43437-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.