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Fluorescent sensors for imaging of interstitial calcium

Author

Listed:
  • Ariel A. Valiente-Gabioud

    (Tools for Bio-Imaging)

  • Inés Garteizgogeascoa Suñer

    (Université Libre de Bruxelles (ULB))

  • Agata Idziak

    (CNRS - Université de Bordeaux − 146 rue Léo-Saignat)

  • Arne Fabritius

    (Tools for Bio-Imaging)

  • Jérome Basquin

    (Max-Planck-Institute for Biochemistry)

  • Julie Angibaud

    (CNRS - Université de Bordeaux − 146 rue Léo-Saignat)

  • U. Valentin Nägerl

    (CNRS - Université de Bordeaux − 146 rue Léo-Saignat)

  • Sumeet Pal Singh

    (Université Libre de Bruxelles (ULB))

  • Oliver Griesbeck

    (Tools for Bio-Imaging)

Abstract

Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues. Here we present high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (Kds) ranging from 0.8 mM to 2.9 mM, making them tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms.

Suggested Citation

  • Ariel A. Valiente-Gabioud & Inés Garteizgogeascoa Suñer & Agata Idziak & Arne Fabritius & Jérome Basquin & Julie Angibaud & U. Valentin Nägerl & Sumeet Pal Singh & Oliver Griesbeck, 2023. "Fluorescent sensors for imaging of interstitial calcium," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41928-w
    DOI: 10.1038/s41467-023-41928-w
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    References listed on IDEAS

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    1. Atsushi Miyawaki & Juan Llopis & Roger Heim & J. Michael McCaffery & Joseph A. Adams & Mitsuhiko Ikura & Roger Y. Tsien, 1997. "Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin," Nature, Nature, vol. 388(6645), pages 882-887, August.
    2. Junji Suzuki & Kazunori Kanemaru & Kuniaki Ishii & Masamichi Ohkura & Yohei Okubo & Masamitsu Iino, 2014. "Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    3. Joachim Goedhart & David von Stetten & Marjolaine Noirclerc-Savoye & Mickaël Lelimousin & Linda Joosen & Mark A. Hink & Laura van Weeren & Theodorus W.J. Gadella & Antoine Royant, 2012. "Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%," Nature Communications, Nature, vol. 3(1), pages 1-9, January.
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