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A single-cell atlas of West African lungfish respiratory system reveals evolutionary adaptations to terrestrialization

Author

Listed:
  • Ruihua Zhang

    (University of Chinese Academy of Sciences
    BGI Research
    BGI Research)

  • Qun Liu

    (BGI Research
    BGI Research
    University of Copenhagen)

  • Shanshan Pan

    (BGI Research
    BGI Research)

  • Yingying Zhang

    (BGI Research
    BGI Research)

  • Yating Qin

    (BGI Research
    BGI Research)

  • Xiao Du

    (BGI Research
    BGI Research
    BGI Research)

  • Zengbao Yuan

    (University of Chinese Academy of Sciences
    BGI Research
    BGI Research)

  • Yongrui Lu

    (Chinese Academy of Sciences)

  • Yue Song

    (BGI Research
    BGI Research)

  • Mengqi Zhang

    (BGI Research)

  • Nannan Zhang

    (BGI Research
    BGI Research)

  • Jie Ma

    (BGI Research
    BGI Research)

  • Zhe Zhang

    (MGI Tech)

  • Xiaodong Jia

    (Liaocheng People’s Hospital)

  • Kun Wang

    (Northwestern Polytechnical University)

  • Shunping He

    (Chinese Academy of Sciences)

  • Shanshan Liu

    (BGI Research
    MGI Tech)

  • Ming Ni

    (BGI Research
    MGI Tech)

  • Xin Liu

    (BGI Research)

  • Xun Xu

    (BGI Research
    BGI Research)

  • Huanming Yang

    (BGI Research)

  • Jian Wang

    (BGI Research)

  • Inge Seim

    (Nanjing Normal University
    Queensland University of Technology)

  • Guangyi Fan

    (BGI Research
    BGI Research
    BGI Research)

Abstract

The six species of lungfish possess both lungs and gills and are the closest extant relatives of tetrapods. Here, we report a single-cell transcriptome atlas of the West African lungfish (Protopterus annectens). This species manifests the most extreme form of terrestrialization, a life history strategy to survive dry periods that can last for years, characterized by dormancy and reversible adaptive changes of the gills and lungs. Our atlas highlights the cell type diversity of the West African lungfish, including gene expression consistent with phenotype changes of terrestrialization. Comparison with terrestrial tetrapods and ray-finned fishes reveals broad homology between the swim bladder and lung cell types as well as shared and idiosyncratic changes of the external gills of the West African lungfish and the internal gills of Atlantic salmon. The single-cell atlas presented here provides a valuable resource for further exploration of the respiratory system evolution in vertebrates and the diversity of lungfish terrestrialization.

Suggested Citation

  • Ruihua Zhang & Qun Liu & Shanshan Pan & Yingying Zhang & Yating Qin & Xiao Du & Zengbao Yuan & Yongrui Lu & Yue Song & Mengqi Zhang & Nannan Zhang & Jie Ma & Zhe Zhang & Xiaodong Jia & Kun Wang & Shun, 2023. "A single-cell atlas of West African lungfish respiratory system reveals evolutionary adaptations to terrestrialization," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41309-3
    DOI: 10.1038/s41467-023-41309-3
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