IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-39930-3.html
   My bibliography  Save this article

Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

Author

Listed:
  • Yayu Guo

    (Beijing Forestry University)

  • Shufang Wang

    (Beijing Forestry University
    Chinese Academy of Sciences)

  • Keji Yu

    (Beijing Forestry University)

  • Hou-Ling Wang

    (Beijing Forestry University)

  • Huimin Xu

    (Beijing Forestry University
    China Agricultural University)

  • Chengwei Song

    (Beijing Forestry University
    Henan University of Science and Technology)

  • Yuanyuan Zhao

    (Beijing Forestry University)

  • Jialong Wen

    (Beijing Forestry University)

  • Chunxiang Fu

    (Chinese Academy of Sciences)

  • Yu Li

    (Chinese Academy of Sciences)

  • Shuizhong Wang

    (Beijing Forestry University)

  • Xi Zhang

    (Beijing Forestry University)

  • Yan Zhang

    (Beijing Forestry University)

  • Yuan Cao

    (Chinese Academy of Forestry)

  • Fenjuan Shao

    (Chinese Academy of Forestry)

  • Xiaohua Wang

    (Chinese Academy of Sciences)

  • Xin Deng

    (Chinese Academy of Sciences)

  • Tong Chen

    (Chinese Academy of Sciences)

  • Qiao Zhao

    (Chinese Academy of Sciences)

  • Lei Li

    (Peking University)

  • Guodong Wang

    (Chinese Academy of Sciences)

  • Paul Grünhofer

    (University of Bonn)

  • Lukas Schreiber

    (University of Bonn)

  • Yue Li

    (Beijing Forestry University)

  • Guoyong Song

    (Beijing Forestry University)

  • Richard A. Dixon

    (Beijing Forestry University
    University of North Texas)

  • Jinxing Lin

    (Beijing Forestry University)

Abstract

The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.

Suggested Citation

  • Yayu Guo & Shufang Wang & Keji Yu & Hou-Ling Wang & Huimin Xu & Chengwei Song & Yuanyuan Zhao & Jialong Wen & Chunxiang Fu & Yu Li & Shuizhong Wang & Xi Zhang & Yan Zhang & Yuan Cao & Fenjuan Shao & X, 2023. "Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39930-3
    DOI: 10.1038/s41467-023-39930-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-39930-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-39930-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Jack P. Wang & Megan L. Matthews & Cranos M. Williams & Rui Shi & Chenmin Yang & Sermsawat Tunlaya-Anukit & Hsi-Chuan Chen & Quanzi Li & Jie Liu & Chien-Yuan Lin & Punith Naik & Ying-Hsuan Sun & Phili, 2018. "Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    2. Nicholas D. Bonawitz & Jeong Im Kim & Yuki Tobimatsu & Peter N. Ciesielski & Nickolas A. Anderson & Eduardo Ximenes & Junko Maeda & John Ralph & Bryon S. Donohoe & Michael Ladisch & Clint Chapple, 2014. "Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant," Nature, Nature, vol. 509(7500), pages 376-380, May.
    3. Shengnan Li & Dexing Lin & Yunwei Zhang & Min Deng & Yongxing Chen & Bin Lv & Boshu Li & Yuan Lei & Yanpeng Wang & Long Zhao & Yueting Liang & Jinxing Liu & Kunling Chen & Zhiyong Liu & Jun Xiao & Jin, 2022. "Genome-edited powdery mildew resistance in wheat without growth penalties," Nature, Nature, vol. 602(7897), pages 455-460, February.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Fuxi Rong & Yusong Lv & Pingchuan Deng & Xia Wu & Yaqi Zhang & Erkui Yue & Yuxin Shen & Sajid Muhammad & Fangrui Ni & Hongwu Bian & Xiangjin Wei & Weijun Zhou & Peisong Hu & Liang Wu, 2024. "Switching action modes of miR408-5p mediates auxin signaling in rice," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ulaganathan, Kandasamy & Goud, Sravanthi & Reddy, Madhavi & Kayalvili, Ulaganathan, 2017. "Genome engineering for breaking barriers in lignocellulosic bioethanol production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 74(C), pages 1080-1107.
    2. Rongrong Zhang & Yu Wu & Xiangru Qu & Wenjuan Yang & Qin Wu & Lin Huang & Qiantao Jiang & Jian Ma & Yazhou Zhang & Pengfei Qi & Guoyue Chen & Yunfeng Jiang & Youliang Zheng & Xiaojie Wang & Yuming Wei, 2024. "The RING-finger ubiquitin E3 ligase TaPIR1 targets TaHRP1 for degradation to suppress chloroplast function," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    3. Mengmeng Li & Zige Yang & Cheng Chang, 2022. "Susceptibility Is New Resistance: Wheat Susceptibility Genes and Exploitation in Resistance Breeding," Agriculture, MDPI, vol. 12(9), pages 1-13, September.
    4. Taikui Zhang & Weichen Huang & Lin Zhang & De-Zhu Li & Ji Qi & Hong Ma, 2024. "Phylogenomic profiles of whole-genome duplications in Poaceae and landscape of differential duplicate retention and losses among major Poaceae lineages," Nature Communications, Nature, vol. 15(1), pages 1-27, December.
    5. Michelle L. Johnson & Bruce A. Hay & Maciej Maselko, 2024. "Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    6. Monika Yadav & Kunwar Paritosh & Aakash Chawade & Nidhi Pareek & Vivekanand Vivekanand, 2018. "Genetic Engineering of Energy Crops to Reduce Recalcitrance and Enhance Biomass Digestibility," Agriculture, MDPI, vol. 8(6), pages 1-15, June.
    7. Tianye Zhang & Chaonan Shi & Haichao Hu & Zhuo Zhang & Ziqiong Wang & Zhiqing Chen & Huimin Feng & Peng Liu & Jun Guo & Qisen Lu & Kaili Zhong & ZhiHui Chen & Jiaqian Liu & Jiancheng Yu & Jianping Che, 2022. "N6-methyladenosine RNA modification promotes viral genomic RNA stability and infection," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    8. Hang Su & Yuanchun Wang & Jin Xu & Ahmad A. Omar & Jude W. Grosser & Milica Calovic & Liyang Zhang & Yu Feng & Christopher A. Vakulskas & Nian Wang, 2023. "Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    9. Qichen Yuan & Xue Gao, 2022. "Multiplex base- and prime-editing with drive-and-process CRISPR arrays," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    10. Fei Shen & Shixiao Xu & Qi Shen & Changwei Bi & Martin A. Lysak, 2023. "The allotetraploid horseradish genome provides insights into subgenome diversification and formation of critical traits," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    11. Zhaojiang Guo & Le Guo & Jianying Qin & Fan Ye & Dan Sun & Qingjun Wu & Shaoli Wang & Neil Crickmore & Xuguo Zhou & Alejandra Bravo & Mario Soberón & Youjun Zhang, 2022. "A single transcription factor facilitates an insect host combating Bacillus thuringiensis infection while maintaining fitness," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39930-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.