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Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

Author

Listed:
  • Yayu Guo

    (Beijing Forestry University)

  • Shufang Wang

    (Beijing Forestry University
    Chinese Academy of Sciences)

  • Keji Yu

    (Beijing Forestry University)

  • Hou-Ling Wang

    (Beijing Forestry University)

  • Huimin Xu

    (Beijing Forestry University
    China Agricultural University)

  • Chengwei Song

    (Beijing Forestry University
    Henan University of Science and Technology)

  • Yuanyuan Zhao

    (Beijing Forestry University)

  • Jialong Wen

    (Beijing Forestry University)

  • Chunxiang Fu

    (Chinese Academy of Sciences)

  • Yu Li

    (Chinese Academy of Sciences)

  • Shuizhong Wang

    (Beijing Forestry University)

  • Xi Zhang

    (Beijing Forestry University)

  • Yan Zhang

    (Beijing Forestry University)

  • Yuan Cao

    (Chinese Academy of Forestry)

  • Fenjuan Shao

    (Chinese Academy of Forestry)

  • Xiaohua Wang

    (Chinese Academy of Sciences)

  • Xin Deng

    (Chinese Academy of Sciences)

  • Tong Chen

    (Chinese Academy of Sciences)

  • Qiao Zhao

    (Chinese Academy of Sciences)

  • Lei Li

    (Peking University)

  • Guodong Wang

    (Chinese Academy of Sciences)

  • Paul Grünhofer

    (University of Bonn)

  • Lukas Schreiber

    (University of Bonn)

  • Yue Li

    (Beijing Forestry University)

  • Guoyong Song

    (Beijing Forestry University)

  • Richard A. Dixon

    (Beijing Forestry University
    University of North Texas)

  • Jinxing Lin

    (Beijing Forestry University)

Abstract

The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.

Suggested Citation

  • Yayu Guo & Shufang Wang & Keji Yu & Hou-Ling Wang & Huimin Xu & Chengwei Song & Yuanyuan Zhao & Jialong Wen & Chunxiang Fu & Yu Li & Shuizhong Wang & Xi Zhang & Yan Zhang & Yuan Cao & Fenjuan Shao & X, 2023. "Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39930-3
    DOI: 10.1038/s41467-023-39930-3
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    1. Jack P. Wang & Megan L. Matthews & Cranos M. Williams & Rui Shi & Chenmin Yang & Sermsawat Tunlaya-Anukit & Hsi-Chuan Chen & Quanzi Li & Jie Liu & Chien-Yuan Lin & Punith Naik & Ying-Hsuan Sun & Phili, 2018. "Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    2. Shengnan Li & Dexing Lin & Yunwei Zhang & Min Deng & Yongxing Chen & Bin Lv & Boshu Li & Yuan Lei & Yanpeng Wang & Long Zhao & Yueting Liang & Jinxing Liu & Kunling Chen & Zhiyong Liu & Jun Xiao & Jin, 2022. "Genome-edited powdery mildew resistance in wheat without growth penalties," Nature, Nature, vol. 602(7897), pages 455-460, February.
    3. Nicholas D. Bonawitz & Jeong Im Kim & Yuki Tobimatsu & Peter N. Ciesielski & Nickolas A. Anderson & Eduardo Ximenes & Junko Maeda & John Ralph & Bryon S. Donohoe & Michael Ladisch & Clint Chapple, 2014. "Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant," Nature, Nature, vol. 509(7500), pages 376-380, May.
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    Cited by:

    1. Fuxi Rong & Yusong Lv & Pingchuan Deng & Xia Wu & Yaqi Zhang & Erkui Yue & Yuxin Shen & Sajid Muhammad & Fangrui Ni & Hongwu Bian & Xiangjin Wei & Weijun Zhou & Peisong Hu & Liang Wu, 2024. "Switching action modes of miR408-5p mediates auxin signaling in rice," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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