IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-39770-1.html
   My bibliography  Save this article

Lymph node targeted multi-epitope subunit vaccine promotes effective immunity to EBV in HLA-expressing mice

Author

Listed:
  • Vijayendra Dasari

    (Berghofer Medical Research Institute)

  • Lisa K. McNeil

    (Elicio Therapeutics, Inc)

  • Kirrilee Beckett

    (Berghofer Medical Research Institute)

  • Matthew Solomon

    (Berghofer Medical Research Institute)

  • George Ambalathingal

    (Berghofer Medical Research Institute)

  • T. Le Thuy

    (Berghofer Medical Research Institute)

  • Archana Panikkar

    (Berghofer Medical Research Institute)

  • Caitlyn Smith

    (Berghofer Medical Research Institute)

  • Martin P. Steinbuck

    (Elicio Therapeutics, Inc)

  • Aniela Jakubowski

    (Elicio Therapeutics, Inc)

  • Lochana M. Seenappa

    (Elicio Therapeutics, Inc)

  • Erica Palmer

    (Elicio Therapeutics, Inc)

  • Jeff Zhang

    (Elicio Therapeutics, Inc)

  • Christopher M. Haqq

    (Elicio Therapeutics, Inc)

  • Peter C. DeMuth

    (Elicio Therapeutics, Inc)

  • Rajiv Khanna

    (Berghofer Medical Research Institute)

Abstract

The recent emergence of a causal link between Epstein-Barr virus (EBV) and multiple sclerosis has generated considerable interest in the development of an effective vaccine against EBV. Here we describe a vaccine formulation based on a lymph node targeting Amphiphile vaccine adjuvant, Amphiphile-CpG, admixed with EBV gp350 glycoprotein and an engineered EBV polyepitope protein that includes 20 CD8+ T cell epitopes from EBV latent and lytic antigens. Potent gp350-specific IgG responses are induced in mice with titers >100,000 in Amphiphile-CpG vaccinated mice. Immunization including Amphiphile-CpG also induces high frequencies of polyfunctional gp350-specific CD4+ T cells and EBV-specific CD8+ T cells that are 2-fold greater than soluble CpG and are maintained for >7 months post immunization. This combination of broad humoral and cellular immunity against multiple viral determinants is likely to provide better protection against primary infection and control of latently infected B cells leading to protection against the development of EBV-associated diseases.

Suggested Citation

  • Vijayendra Dasari & Lisa K. McNeil & Kirrilee Beckett & Matthew Solomon & George Ambalathingal & T. Le Thuy & Archana Panikkar & Caitlyn Smith & Martin P. Steinbuck & Aniela Jakubowski & Lochana M. Se, 2023. "Lymph node targeted multi-epitope subunit vaccine promotes effective immunity to EBV in HLA-expressing mice," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39770-1
    DOI: 10.1038/s41467-023-39770-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-39770-1
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-39770-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Tobias V. Lanz & R. Camille Brewer & Peggy P. Ho & Jae-Seung Moon & Kevin M. Jude & Daniel Fernandez & Ricardo A. Fernandes & Alejandro M. Gomez & Gabriel-Stefan Nadj & Christopher M. Bartley & Ryan D, 2022. "Clonally expanded B cells in multiple sclerosis bind EBV EBNA1 and GlialCAM," Nature, Nature, vol. 603(7900), pages 321-327, March.
    2. Haipeng Liu & Kelly D. Moynihan & Yiran Zheng & Gregory L. Szeto & Adrienne V. Li & Bonnie Huang & Debra S. Van Egeren & Clara Park & Darrell J. Irvine, 2014. "Structure-based programming of lymph-node targeting in molecular vaccines," Nature, Nature, vol. 507(7493), pages 519-522, March.
    3. Adam E Handel & Alexander J Williamson & Giulio Disanto & Lahiru Handunnetthi & Gavin Giovannoni & Sreeram V Ramagopalan, 2010. "An Updated Meta-Analysis of Risk of Multiple Sclerosis following Infectious Mononucleosis," PLOS ONE, Public Library of Science, vol. 5(9), pages 1-5, September.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jia He & Chaoyu Wang & Xiao Fang & Junyao Li & Xueying Shen & Junxia Zhang & Cheng Peng & Hongjian Li & Sai Li & Jeffrey M. Karp & Rui Kuai, 2024. "Tuning the fluidity and protein corona of ultrasound-responsive liposomal nanovaccines to program T cell immunity in mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Ella N. Hoogenboezem & Shrusti S. Patel & Justin H. Lo & Ashley B. Cavnar & Lauren M. Babb & Nora Francini & Eva F. Gbur & Prarthana Patil & Juan M. Colazo & Danielle L. Michell & Violeta M. Sanchez &, 2024. "Structural optimization of siRNA conjugates for albumin binding achieves effective MCL1-directed cancer therapy," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    3. Grant Broussard & Guoxin Ni & Zhigang Zhang & Qian Li & Patricio Cano & Dirk P. Dittmer & Blossom Damania, 2023. "Barrier-to-autointegration factor 1 promotes gammaherpesvirus reactivation from latency," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Brian D. Ross & Youngsoon Jang & Amanda Welton & Christopher A. Bonham & Dilrukshika S. W. Palagama & Kevin Heist & Jagadish Boppisetti & Kasun P. Imaduwage & Tanner Robison & Leah R. King & Edward Z., 2022. "A lymphatic-absorbed multi-targeted kinase inhibitor for myelofibrosis therapy," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    5. Shenqiang Wang & Ying Zhang & Yanfang Wang & Yinxian Yang & Sheng Zhao & Tao Sheng & Yuqi Zhang & Zhen Gu & Jinqiang Wang & Jicheng Yu, 2023. "An in situ dual-anchoring strategy for enhanced immobilization of PD-L1 to treat autoimmune diseases," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    6. Garrett Dunlap & Aaron Wagner & Nida Meednu & Ruoqiao Wang & Fan Zhang & Jabea Cyril Ekabe & Anna Helena Jonsson & Kevin Wei & Saori Sakaue & Aparna Nathan & Vivian P. Bykerk & Laura T. Donlin & Susan, 2024. "Clonal associations between lymphocyte subsets and functional states in rheumatoid arthritis synovium," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39770-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.