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The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation

Author

Listed:
  • Zheng Li

    (University of California)

  • Ming Wang

    (University of California)

  • Zhenhui Zhong

    (University of California)

  • Javier Gallego-Bartolomé

    (University of California
    CSIC-Universitat Politècnica de València)

  • Suhua Feng

    (University of California
    University of California at Los Angeles)

  • Yasaman Jami-Alahmadi

    (University of California)

  • Xinyi Wang

    (University of California)

  • James Wohlschlegel

    (University of California)

  • Sylvain Bischof

    (University of California
    University of Zurich)

  • Jeff A. Long

    (University of California)

  • Steven E. Jacobsen

    (University of California
    University of California at Los Angeles
    University of California)

Abstract

MORPHEUS’ MOLECULE1 (MOM1) is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we find that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with an artificial zinc finger to an unmethylated FWA promoter leads to establishment of DNA methylation and FWA silencing. This process is blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MICRORCHIDIA 6 (MORC6). We find that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes is impaired in the mom1 mutant. In addition to RdDM sites, we identify a group of MOM1 peaks at active chromatin near genes that colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation.

Suggested Citation

  • Zheng Li & Ming Wang & Zhenhui Zhong & Javier Gallego-Bartolomé & Suhua Feng & Yasaman Jami-Alahmadi & Xinyi Wang & James Wohlschlegel & Sylvain Bischof & Jeff A. Long & Steven E. Jacobsen, 2023. "The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39751-4
    DOI: 10.1038/s41467-023-39751-4
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    1. Linhua Sun & Jingru Zhou & Xiao Xu & Yi Liu & Ni Ma & Yutong Liu & Wenchao Nie & Ling Zou & Xing Wang Deng & Hang He, 2024. "Mapping nucleosome-resolution chromatin organization and enhancer-promoter loops in plants using Micro-C-XL," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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