IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-39011-5.html
   My bibliography  Save this article

The long non-coding RNA NEAT1 is a ΔNp63 target gene modulating epidermal differentiation

Author

Listed:
  • Claudia Fierro

    (University of Rome “Tor Vergata”
    Bambino Gesù Children’s Hospital, IRCSS)

  • Veronica Gatti

    (Institute of Translational Pharmacology (IFT), CNR)

  • Veronica Banca

    (University of Rome “Tor Vergata”)

  • Sara Domenico

    (University of Rome “Tor Vergata”)

  • Stefano Scalera

    (Advanced Diagnostics, and Technological Innovation, Translational Research Area, IRCCS Regina Elena National Cancer Institute)

  • Giacomo Corleone

    (Advanced Diagnostics, and Technological Innovation, Translational Research Area, IRCCS Regina Elena National Cancer Institute)

  • Maurizio Fanciulli

    (Advanced Diagnostics, and Technological Innovation, Translational Research Area, IRCCS Regina Elena National Cancer Institute)

  • Francesca Nicola

    (Advanced Diagnostics, and Technological Innovation, Translational Research Area, IRCCS Regina Elena National Cancer Institute)

  • Alessandro Mauriello

    (University of Rome “Tor Vergata”)

  • Manuela Montanaro

    (University of Rome “Tor Vergata”)

  • George A. Calin

    (The University of Texas MD Anderson Cancer Center
    The University of Texas MD Anderson Cancer Center)

  • Gerry Melino

    (University of Rome “Tor Vergata”)

  • Angelo Peschiaroli

    (Institute of Translational Pharmacology (IFT), CNR)

Abstract

The transcription factor ΔNp63 regulates epithelial stem cell function and maintains the integrity of stratified epithelial tissues by acting as transcriptional repressor or activator towards a distinct subset of protein-coding genes and microRNAs. However, our knowledge of the functional link between ∆Np63 transcriptional activity and long non-coding RNAs (lncRNAs) expression is quite limited. Here, we show that in proliferating human keratinocytes ∆Np63 represses the expression of the lncRNA NEAT1 by recruiting the histone deacetylase HDAC1 to the proximal promoter of NEAT1 genomic locus. Upon induction of differentiation, ∆Np63 down-regulation is associated by a marked increase of NEAT1 RNA levels, resulting in an increased assembly of paraspeckles foci both in vitro and in human skin tissues. RNA-seq analysis associated with global DNA binding profile (ChIRP-seq) revealed that NEAT1 associates with the promoter of key epithelial transcription factors sustaining their expression during epidermal differentiation. These molecular events might explain the inability of NEAT1-depleted keratinocytes to undergo the proper formation of epidermal layers. Collectively, these data uncover the lncRNA NEAT1 as an additional player of the intricate network orchestrating epidermal morphogenesis.

Suggested Citation

  • Claudia Fierro & Veronica Gatti & Veronica Banca & Sara Domenico & Stefano Scalera & Giacomo Corleone & Maurizio Fanciulli & Francesca Nicola & Alessandro Mauriello & Manuela Montanaro & George A. Cal, 2023. "The long non-coding RNA NEAT1 is a ΔNp63 target gene modulating epidermal differentiation," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39011-5
    DOI: 10.1038/s41467-023-39011-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-39011-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-39011-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Dimple Chakravarty & Andrea Sboner & Sujit S. Nair & Eugenia Giannopoulou & Ruohan Li & Sven Hennig & Juan Miguel Mosquera & Jonathan Pauwels & Kyung Park & Myriam Kossai & Theresa Y. MacDonald & Jacq, 2014. "The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer," Nature Communications, Nature, vol. 5(1), pages 1-16, December.
    2. Fulai Jin & Yan Li & Jesse R. Dixon & Siddarth Selvaraj & Zhen Ye & Ah Young Lee & Chia-An Yen & Anthony D. Schmitt & Celso A. Espinoza & Bing Ren, 2013. "A high-resolution map of the three-dimensional chromatin interactome in human cells," Nature, Nature, vol. 503(7475), pages 290-294, November.
    3. Annie Yang & Ronen Schweitzer & Deqin Sun & Mourad Kaghad & Nancy Walker & Roderick T. Bronson & Cliff Tabin & Arlene Sharpe & Daniel Caput & Christopher Crum & Frank McKeon, 1999. "p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development," Nature, Nature, vol. 398(6729), pages 714-718, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Hye Ji Cha & Özgün Uyan & Yan Kai & Tianxin Liu & Qian Zhu & Zuzana Tothova & Giovanni A. Botten & Jian Xu & Guo-Cheng Yuan & Job Dekker & Stuart H. Orkin, 2021. "Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    2. Zhang Qi & Xu Zheng & Lai Yutong, 2021. "An Empirical Bayes approach for the identification of long-range chromosomal interaction from Hi-C data," Statistical Applications in Genetics and Molecular Biology, De Gruyter, vol. 20(1), pages 1-15, February.
    3. Zhaohui Qin & Ben Li & Karen N. Conneely & Hao Wu & Ming Hu & Deepak Ayyala & Yongseok Park & Victor X. Jin & Fangyuan Zhang & Han Zhang & Li Li & Shili Lin, 2016. "Statistical Challenges in Analyzing Methylation and Long-Range Chromosomal Interaction Data," Statistics in Biosciences, Springer;International Chinese Statistical Association, vol. 8(2), pages 284-309, October.
    4. David Santiago-Algarra & Charbel Souaid & Himanshu Singh & Lan T. M. Dao & Saadat Hussain & Alejandra Medina-Rivera & Lucia Ramirez-Navarro & Jaime A. Castro-Mondragon & Nori Sadouni & Guillaume Charb, 2021. "Epromoters function as a hub to recruit key transcription factors required for the inflammatory response," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    5. Ofir Shukron & David Holcman, 2017. "Transient chromatin properties revealed by polymer models and stochastic simulations constructed from Chromosomal Capture data," PLOS Computational Biology, Public Library of Science, vol. 13(4), pages 1-20, April.
    6. David E. Torres & H. Martin Kramer & Vittorio Tracanna & Gabriel L. Fiorin & David E. Cook & Michael F. Seidl & Bart P. H. J. Thomma, 2024. "Implications of the three-dimensional chromatin organization for genome evolution in a fungal plant pathogen," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    7. Hisato Nagano & Naoaki Mizuno & Hideyuki Sato & Eiji Mizutani & Ayaka Yanagida & Mayuko Kano & Mariko Kasai & Hiromi Yamamoto & Motoo Watanabe & Fabian Suchy & Hideki Masaki & Hiromitsu Nakauchi, 2024. "Skin graft with dermis and appendages generated in vivo by cell competition," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39011-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.