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High-resolution discrimination of homologous and isomeric proteinogenic amino acids in nanopore sensors with ultrashort single-walled carbon nanotubes

Author

Listed:
  • Weichao Peng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Shuaihu Yan

    (University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Ke Zhou

    (Chinese Academy of Sciences)

  • Hai-Chen Wu

    (University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Lei Liu

    (Chinese Academy of Sciences)

  • Yuliang Zhao

    (Chinese Academy of Sciences
    National Center for Nanoscience and Technology)

Abstract

The hollow and tubular structure of single-walled carbon nanotubes (SWCNTs) makes them ideal candidates for making nanopores. However, the heterogeneity of SWCNTs hinders the fabrication of robust and reproducible carbon-based nanopore sensors. Here we develop a modified density gradient ultracentrifugation approach to separate ultrashort (≈5-10 nm) SWCNTs with a narrow conductance range and construct high-resolution nanopore sensors with those tubes inserted in lipid bilayers. By conducting ionic current recordings and fluorescent imaging of Ca2+ flux through different nanopores, we prove that the ion mobilities in SWCNT nanopores are 3-5 times higher than the bulk mobility. Furthermore, we employ SWCNT nanopores to discriminate homologue or isomeric proteinogenic amino acids, which are challenging tasks for other nanopore sensors. These successes, coupled with the building of SWCNT nanopore arrays, may constitute a crucial part of the recently burgeoning protein sequencing technologies.

Suggested Citation

  • Weichao Peng & Shuaihu Yan & Ke Zhou & Hai-Chen Wu & Lei Liu & Yuliang Zhao, 2023. "High-resolution discrimination of homologous and isomeric proteinogenic amino acids in nanopore sensors with ultrashort single-walled carbon nanotubes," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38399-4
    DOI: 10.1038/s41467-023-38399-4
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    References listed on IDEAS

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    1. G. Hummer & J. C. Rasaiah & J. P. Noworyta, 2001. "Water conduction through the hydrophobic channel of a carbon nanotube," Nature, Nature, vol. 414(6860), pages 188-190, November.
    2. Hagan Bayley & Paul S. Cremer, 2001. "Stochastic sensors inspired by biology," Nature, Nature, vol. 413(6852), pages 226-230, September.
    3. Jia Geng & Kyunghoon Kim & Jianfei Zhang & Artur Escalada & Ramya Tunuguntla & Luis R. Comolli & Frances I. Allen & Anna V. Shnyrova & Kang Rae Cho & Dayannara Munoz & Y. Morris Wang & Costas P. Grigo, 2014. "Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes," Nature, Nature, vol. 514(7524), pages 612-615, October.
    4. Yunhyun Lee & Hyun Jung Kim & Dong-Kwon Kim, 2020. "Power Generation from Concentration Gradient by Reverse Electrodialysis in Anisotropic Nanoporous Anodic Aluminum Oxide Membranes," Energies, MDPI, vol. 13(4), pages 1-15, February.
    5. Lei Liu & Chun Yang & Kai Zhao & Jingyuan Li & Hai-Chen Wu, 2013. "Ultrashort single-walled carbon nanotubes in a lipid bilayer as a new nanopore sensor," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    6. Jhinhwan Lee & H. Kim & S.-J. Kahng & G. Kim & Y.-W. Son & J. Ihm & H. Kato & Z. W. Wang & T. Okazaki & H. Shinohara & Young Kuk, 2002. "Bandgap modulation of carbon nanotubes by encapsulated metallofullerenes," Nature, Nature, vol. 415(6875), pages 1005-1008, February.
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