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DNMT1 mutant ants develop normally but have disrupted oogenesis

Author

Listed:
  • Iryna Ivasyk

    (The Rockefeller University)

  • Leonora Olivos-Cisneros

    (The Rockefeller University)

  • Stephany Valdés-Rodríguez

    (The Rockefeller University
    Howard Hughes Medical Institute)

  • Marie Droual

    (The Rockefeller University)

  • Hosung Jang

    (University of Georgia)

  • Robert J. Schmitz

    (University of Georgia)

  • Daniel J. C. Kronauer

    (The Rockefeller University
    Howard Hughes Medical Institute)

Abstract

Although DNA methylation is an important gene regulatory mechanism in mammals, its function in arthropods remains poorly understood. Studies in eusocial insects have argued for its role in caste development by regulating gene expression and splicing. However, such findings are not always consistent across studies, and have therefore remained controversial. Here we use CRISPR/Cas9 to mutate the maintenance DNA methyltransferase DNMT1 in the clonal raider ant, Ooceraea biroi. Mutants have greatly reduced DNA methylation, but no obvious developmental phenotypes, demonstrating that, unlike mammals, ants can undergo normal development without DNMT1 or DNA methylation. Additionally, we find no evidence of DNA methylation regulating caste development. However, mutants are sterile, whereas in wild-type ants, DNMT1 is localized to the ovaries and maternally provisioned into nascent oocytes. This supports the idea that DNMT1 plays a crucial but unknown role in the insect germline.

Suggested Citation

  • Iryna Ivasyk & Leonora Olivos-Cisneros & Stephany Valdés-Rodríguez & Marie Droual & Hosung Jang & Robert J. Schmitz & Daniel J. C. Kronauer, 2023. "DNMT1 mutant ants develop normally but have disrupted oogenesis," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37945-4
    DOI: 10.1038/s41467-023-37945-4
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    References listed on IDEAS

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    1. Matthew D. Schultz & Yupeng He & John W. Whitaker & Manoj Hariharan & Eran A. Mukamel & Danny Leung & Nisha Rajagopal & Joseph R. Nery & Mark A. Urich & Huaming Chen & Shin Lin & Yiing Lin & Inkyung J, 2015. "Human body epigenome maps reveal noncanonical DNA methylation variation," Nature, Nature, vol. 523(7559), pages 212-216, July.
    2. Sebastian Alvarado & Rajendhran Rajakumar & Ehab Abouheif & Moshe Szyf, 2015. "Epigenetic variation in the Egfr gene generates quantitative variation in a complex trait in ants," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    3. Serafino Teseo & Nicolas Châline & Pierre Jaisson & Daniel J.C. Kronauer, 2014. "Epistasis between adults and larvae underlies caste fate and fitness in a clonal ant," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
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    Cited by:

    1. Peter Sarkies & Jennifer Westoby & Rebecca Mary Kilner & Rahia Mashoodh, 2024. "Gene body methylation evolves during the sustained loss of parental care in the burying beetle," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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