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Cryo-EM structures of mitochondrial ABC transporter ABCB10 in apo and biliverdin-bound form

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  • Sheng Cao

    (Wuxi Biortus Biosciences Co. Ltd.)

  • Yihu Yang

    (Wuxi Biortus Biosciences Co. Ltd.)

  • Lili He

    (Tongji Medical College, Huazhong University of Science and Technology)

  • Yumo Hang

    (Tongji Medical College, Huazhong University of Science and Technology)

  • Xiaodong Yan

    (Wuxi Biortus Biosciences Co. Ltd.)

  • Hui Shi

    (Wuxi Biortus Biosciences Co. Ltd.)

  • Jiaquan Wu

    (Wuxi Biortus Biosciences Co. Ltd.)

  • Zhuqing Ouyang

    (Tongji Medical College, Huazhong University of Science and Technology)

Abstract

ABCB10, a member of ABC transporter superfamily that locates in the inner membrane of mitochondria, plays crucial roles in hemoglobin synthesis, antioxidative stress and stabilization of the iron transporter mitoferrin-1. Recently, it was found that ABCB10 is a mitochondrial biliverdin exporter. However, the molecular mechanism of biliverdin export by ABCB10 remains elusive. Here we report the cryo-EM structures of ABCB10 in apo (ABCB10-apo) and biliverdin-bound form (ABCB10-BV) at 3.67 Å and 2.85 Å resolution, respectively. ABCB10-apo adopts a wide-open conformation and may thus represent the apo form structure. ABCB10-BV forms a closed conformation and biliverdin situates in a hydrophobic pocket in one protomer and bridges the interaction through hydrogen bonds with the opposing one. We also identify cholesterols sandwiched by BVs and discuss the export dynamics based on these structural and biochemical observations.

Suggested Citation

  • Sheng Cao & Yihu Yang & Lili He & Yumo Hang & Xiaodong Yan & Hui Shi & Jiaquan Wu & Zhuqing Ouyang, 2023. "Cryo-EM structures of mitochondrial ABC transporter ABCB10 in apo and biliverdin-bound form," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37851-9
    DOI: 10.1038/s41467-023-37851-9
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    1. Wei Mi & Yanyan Li & Sung Hwan Yoon & Robert K. Ernst & Thomas Walz & Maofu Liao, 2017. "Structural basis of MsbA-mediated lipopolysaccharide transport," Nature, Nature, vol. 549(7671), pages 233-237, September.
    2. Roger J. P. Dawson & Kaspar P. Locher, 2006. "Structure of a bacterial multidrug ABC transporter," Nature, Nature, vol. 443(7108), pages 180-185, September.
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