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Ripply suppresses Tbx6 to induce dynamic-to-static conversion in somite segmentation

Author

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  • Taijiro Yabe

    (National Institutes of Natural Sciences
    National Institutes of Natural Sciences
    The Graduate University for Advanced Studies (SOKENDAI))

  • Koichiro Uriu

    (Kanazawa University, Kakuma-machi)

  • Shinji Takada

    (National Institutes of Natural Sciences
    National Institutes of Natural Sciences
    The Graduate University for Advanced Studies (SOKENDAI))

Abstract

The metameric pattern of somites is created based on oscillatory expression of clock genes in presomitic mesoderm. However, the mechanism for converting the dynamic oscillation to a static pattern of somites is still unclear. Here, we provide evidence that Ripply/Tbx6 machinery is a key regulator of this conversion. Ripply1/Ripply2-mediated removal of Tbx6 protein defines somite boundary and also leads to cessation of clock gene expression in zebrafish embryos. On the other hand, activation of ripply1/ripply2 mRNA and protein expression is periodically regulated by clock oscillation in conjunction with an Erk signaling gradient. Whereas Ripply protein decreases rapidly in embryos, Ripply-triggered Tbx6 suppression persists long enough to complete somite boundary formation. Mathematical modeling shows that a molecular network based on results of this study can reproduce dynamic-to-static conversion in somitogenesis. Furthermore, simulations with this model suggest that sustained suppression of Tbx6 caused by Ripply is crucial in this conversion.

Suggested Citation

  • Taijiro Yabe & Koichiro Uriu & Shinji Takada, 2023. "Ripply suppresses Tbx6 to induce dynamic-to-static conversion in somite segmentation," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37745-w
    DOI: 10.1038/s41467-023-37745-w
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    References listed on IDEAS

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    1. Margarete Diaz-Cuadros & Daniel E. Wagner & Christoph Budjan & Alexis Hubaud & Oscar A. Tarazona & Sophia Donelly & Arthur Michaut & Ziad Al Tanoury & Kumiko Yoshioka-Kobayashi & Yusuke Niino & Ryoich, 2020. "In vitro characterization of the human segmentation clock," Nature, Nature, vol. 580(7801), pages 113-118, April.
    2. François Giudicelli & Ertuğrul M Özbudak & Gavin J Wright & Julian Lewis, 2007. "Setting the Tempo in Development: An Investigation of the Zebrafish Somite Clock Mechanism," PLOS Biology, Public Library of Science, vol. 5(6), pages 1-15, May.
    3. Kumiko Yoshioka-Kobayashi & Marina Matsumiya & Yusuke Niino & Akihiro Isomura & Hiroshi Kori & Atsushi Miyawaki & Ryoichiro Kageyama, 2020. "Coupling delay controls synchronized oscillation in the segmentation clock," Nature, Nature, vol. 580(7801), pages 119-123, April.
    4. Julien Dubrulle & Olivier Pourquié, 2004. "fgf8 mRNA decay establishes a gradient that couples axial elongation to patterning in the vertebrate embryo," Nature, Nature, vol. 427(6973), pages 419-422, January.
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