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Turning universal O into rare Bombay type blood

Author

Listed:
  • Itxaso Anso

    (Cruces University Hospital
    Basque Research and Technology Alliance (BRTA))

  • Andreas Naegeli

    (Genovis AB)

  • Javier O. Cifuente

    (Cruces University Hospital
    Basque Research and Technology Alliance (BRTA))

  • Ane Orrantia

    (Cruces University Hospital)

  • Erica Andersson

    (Genovis AB)

  • Olatz Zenarruzabeitia

    (Cruces University Hospital)

  • Alicia Moraleda-Montoya

    (Cruces University Hospital)

  • Mikel García-Alija

    (Cruces University Hospital
    Basque Research and Technology Alliance (BRTA))

  • Francisco Corzana

    (Universidad de La Rioja)

  • Rafael A. Orbe

    (Biocruces Bizkaia Health Research Institute)

  • Francisco Borrego

    (Cruces University Hospital
    Basque Foundation for Science)

  • Beatriz Trastoy

    (Cruces University Hospital
    Basque Research and Technology Alliance (BRTA)
    Basque Foundation for Science)

  • Jonathan Sjögren

    (Genovis AB)

  • Marcelo E. Guerin

    (Cruces University Hospital
    Basque Research and Technology Alliance (BRTA)
    Basque Foundation for Science)

Abstract

Red blood cell antigens play critical roles in blood transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency in H antigen, the Oh Bombay phenotype, can only be transfused with group Oh blood to avoid serious transfusion reactions. We discover FucOB from the mucin-degrading bacteria Akkermansia muciniphila as an α-1,2-fucosidase able to hydrolyze Type I, Type II, Type III and Type V H antigens to obtain the afucosylated Bombay phenotype in vitro. X-ray crystal structures of FucOB show a three-domain architecture, including a GH95 glycoside hydrolase. The structural data together with site-directed mutagenesis, enzymatic activity and computational methods provide molecular insights into substrate specificity and catalysis. Furthermore, using agglutination tests and flow cytometry-based techniques, we demonstrate the ability of FucOB to convert universal O type into rare Bombay type blood, providing exciting possibilities to facilitate transfusion in recipients/patients with Bombay phenotype.

Suggested Citation

  • Itxaso Anso & Andreas Naegeli & Javier O. Cifuente & Ane Orrantia & Erica Andersson & Olatz Zenarruzabeitia & Alicia Moraleda-Montoya & Mikel García-Alija & Francisco Corzana & Rafael A. Orbe & Franci, 2023. "Turning universal O into rare Bombay type blood," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37324-z
    DOI: 10.1038/s41467-023-37324-z
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    References listed on IDEAS

    as
    1. Beatriz Trastoy & Andreas Naegeli & Itxaso Anso & Jonathan Sjögren & Marcelo E. Guerin, 2020. "Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    2. Artur Rogowski & Jonathon A. Briggs & Jennifer C. Mortimer & Theodora Tryfona & Nicolas Terrapon & Elisabeth C. Lowe & Arnaud Baslé & Carl Morland & Alison M. Day & Hongjun Zheng & Theresa E. Rogers &, 2015. "Glycan complexity dictates microbial resource allocation in the large intestine," Nature Communications, Nature, vol. 6(1), pages 1-16, November.
    3. Plinio S. Vieira & Isabela M. Bonfim & Evandro A. Araujo & Ricardo R. Melo & Augusto R. Lima & Melissa R. Fessel & Douglas A. A. Paixão & Gabriela F. Persinoti & Silvana A. Rocco & Tatiani B. Lima & R, 2021. "Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    4. Michael Jakob Pichler & Chihaya Yamada & Bashar Shuoker & Camila Alvarez-Silva & Aina Gotoh & Maria Louise Leth & Erwin Schoof & Toshihiko Katoh & Mikiyasu Sakanaka & Takane Katayama & Chunsheng Jin &, 2020. "Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    5. Beatriz Trastoy & Erik Klontz & Jared Orwenyo & Alberto Marina & Lai-Xi Wang & Eric J. Sundberg & Marcelo E. Guerin, 2018. "Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
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