IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37265-7.html
   My bibliography  Save this article

Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci

Author

Listed:
  • Inês Catarina Ramos Barbosa

    (University of Lausanne)

  • Damien De Bellis

    (University of Lausanne)

  • Isabelle Flückiger

    (University of Lausanne)

  • Etienne Bellani

    (University of Lausanne)

  • Mathieu Grangé-Guerment

    (University of Lausanne)

  • Kian Hématy

    (University of Lausanne
    Université Paris-Saclay)

  • Niko Geldner

    (University of Lausanne)

Abstract

Casparian strips (CS) are aligned bands of lignin-impregnated cell walls, building an extracellular diffusion barrier in roots. Their structure profoundly differs from tight junctions (TJ), analogous structures in animals. Nonetheless, CS membrane domain (CSD) proteins 1-5 (CASP1-5) are homologues of occludins, TJ components. CASP-marked membranes display cell wall (matrix) adhesion and membrane protein exclusion. A full CASP knock-out now reveals CASPs are not needed for localized lignification, since correctly positioned lignin microdomains still form in the mutant. Ultra-structurally, however, these microdomains are disorganized, showing excessive cell wall growth, lack of exclusion zone and matrix adhesion, and impaired exocyst dynamics. Proximity-labelling identifies a Rab-GTPase subfamily, known exocyst activators, as potential CASP-interactors and demonstrate their localization and function at the CSD. We propose that CASP microdomains displace initial secretory foci by excluding vesicle tethering factors, thereby ensuring rapid fusion of microdomains into a membrane-cell wall band that seals the extracellular space.

Suggested Citation

  • Inês Catarina Ramos Barbosa & Damien De Bellis & Isabelle Flückiger & Etienne Bellani & Mathieu Grangé-Guerment & Kian Hématy & Niko Geldner, 2023. "Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37265-7
    DOI: 10.1038/s41467-023-37265-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37265-7
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-37265-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Damien De Bellis & Lothar Kalmbach & Peter Marhavy & Jean Daraspe & Niko Geldner & Marie Barberon, 2022. "Extracellular vesiculo-tubular structures associated with suberin deposition in plant cell walls," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Daniele Roppolo & Bert De Rybel & Valérie Dénervaud Tendon & Alexandre Pfister & Julien Alassimone & Joop E. M. Vermeer & Misako Yamazaki & York-Dieter Stierhof & Tom Beeckman & Niko Geldner, 2011. "A novel protein family mediates Casparian strip formation in the endodermis," Nature, Nature, vol. 473(7347), pages 380-383, May.
    3. Guilhem Reyt & Priya Ramakrishna & Isai Salas-González & Satoshi Fujita & Ashley Love & David Tiemessen & Catherine Lapierre & Kris Morreel & Monica Calvo-Polanco & Paulina Flis & Niko Geldner & Yann , 2021. "Two chemically distinct root lignin barriers control solute and water balance," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    4. Syed Mukhtar Ahmed & Hisayo Nishida-Fukuda & Yuchong Li & W. Hayes McDonald & Claudiu C. Gradinaru & Ian G. Macara, 2018. "Exocyst dynamics during vesicle tethering and fusion," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zahra Zahra & Zunaira Habib & Hyeseung Hyun & Hafiz Muhammad Aamir Shahzad, 2022. "Overview on Recent Developments in the Design, Application, and Impacts of Nanofertilizers in Agriculture," Sustainability, MDPI, vol. 14(15), pages 1-19, August.
    2. Hongxian Zhu & Andrew M. Sydor & Kirsten C. Boddy & Etienne Coyaud & Estelle M. N. Laurent & Aaron Au & Joel M. J. Tan & Bing-Ru Yan & Jason Moffat & Aleixo M. Muise & Christopher M. Yip & Sergio Grin, 2024. "Salmonella exploits membrane reservoirs for invasion of host cells," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Di-Ao Liu & Kai Tao & Bin Wu & Ziyan Yu & Malwina Szczepaniak & Matthew Rames & Changsong Yang & Tatyana Svitkina & Yueyao Zhu & Fengyuan Xu & Xiaolin Nan & Wei Guo, 2023. "A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Damien De Bellis & Lothar Kalmbach & Peter Marhavy & Jean Daraspe & Niko Geldner & Marie Barberon, 2022. "Extracellular vesiculo-tubular structures associated with suberin deposition in plant cell walls," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    5. Yanyan Wang & Yibo Cao & Xiaoyan Liang & Junhong Zhuang & Xiangfeng Wang & Feng Qin & Caifu Jiang, 2022. "A dirigent family protein confers variation of Casparian strip thickness and salt tolerance in maize," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37265-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.