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Exocyst dynamics during vesicle tethering and fusion

Author

Listed:
  • Syed Mukhtar Ahmed

    (Vanderbilt University School of Medicine)

  • Hisayo Nishida-Fukuda

    (Vanderbilt University School of Medicine
    Ehime University Graduate School of Medicine
    Ehime University Graduate School of Medicine
    Kansai Medical University)

  • Yuchong Li

    (University of Toronto
    University of Toronto Mississauga)

  • W. Hayes McDonald

    (Vanderbilt University School of Medicine)

  • Claudiu C. Gradinaru

    (University of Toronto
    University of Toronto Mississauga)

  • Ian G. Macara

    (Vanderbilt University School of Medicine)

Abstract

The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma membrane prior to fusion. Exocyst assembly and delivery mechanisms remain unclear, especially in mammalian cells. Here we tagged multiple endogenous exocyst subunits with sfGFP or Halo using Cas9 gene-editing, to create single and double knock-in lines of mammary epithelial cells, and interrogated exocyst dynamics by high-speed imaging and correlation spectroscopy. We discovered that mammalian exocyst is comprised of tetrameric subcomplexes that can associate independently with vesicles and plasma membrane and are in dynamic equilibrium with octamer and monomers. Membrane arrival times are similar for subunits and vesicles, but with a small delay (~80msec) between subcomplexes. Departure of SEC3 occurs prior to fusion, whereas other subunits depart just after fusion. About 9 exocyst complexes are associated per vesicle. These data reveal the mammalian exocyst as a remarkably dynamic two-part complex and provide important insights into assembly/disassembly mechanisms.

Suggested Citation

  • Syed Mukhtar Ahmed & Hisayo Nishida-Fukuda & Yuchong Li & W. Hayes McDonald & Claudiu C. Gradinaru & Ian G. Macara, 2018. "Exocyst dynamics during vesicle tethering and fusion," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07467-5
    DOI: 10.1038/s41467-018-07467-5
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    Cited by:

    1. Di-Ao Liu & Kai Tao & Bin Wu & Ziyan Yu & Malwina Szczepaniak & Matthew Rames & Changsong Yang & Tatyana Svitkina & Yueyao Zhu & Fengyuan Xu & Xiaolin Nan & Wei Guo, 2023. "A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Inês Catarina Ramos Barbosa & Damien De Bellis & Isabelle Flückiger & Etienne Bellani & Mathieu Grangé-Guerment & Kian Hématy & Niko Geldner, 2023. "Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Hongxian Zhu & Andrew M. Sydor & Kirsten C. Boddy & Etienne Coyaud & Estelle M. N. Laurent & Aaron Au & Joel M. J. Tan & Bing-Ru Yan & Jason Moffat & Aleixo M. Muise & Christopher M. Yip & Sergio Grin, 2024. "Salmonella exploits membrane reservoirs for invasion of host cells," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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