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Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases

Author

Listed:
  • Eirik G. Kommedal

    (Norwegian University of Life Sciences (NMBU))

  • Camilla F. Angeltveit

    (Norwegian University of Life Sciences (NMBU))

  • Leesa J. Klau

    (Norwegian University of Science and Technology (NTNU))

  • Iván Ayuso-Fernández

    (Norwegian University of Life Sciences (NMBU))

  • Bjørnar Arstad

    (Process Chemistry and Functional Materials)

  • Simen G. Antonsen

    (Norwegian University of Life Sciences (NMBU))

  • Yngve Stenstrøm

    (Norwegian University of Life Sciences (NMBU))

  • Dag Ekeberg

    (Norwegian University of Life Sciences (NMBU))

  • Francisco Gírio

    (National Laboratory of Energy and Geology (LNEG))

  • Florbela Carvalheiro

    (National Laboratory of Energy and Geology (LNEG))

  • Svein J. Horn

    (Norwegian University of Life Sciences (NMBU))

  • Finn Lillelund Aachmann

    (Norwegian University of Science and Technology (NTNU))

  • Vincent G. H. Eijsink

    (Norwegian University of Life Sciences (NMBU))

Abstract

Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of plant biomass in Nature and in industrial applications. Sunlight promotes microbial conversion of plant litter; this effect has been attributed to photochemical degradation of lignin, a major redox-active component of secondary plant cell walls that limits enzyme access to the cell wall carbohydrates. Here, we show that exposing lignin to visible light facilitates cellulose solubilization by promoting formation of H2O2 that fuels LPMO catalysis. Light-driven H2O2 formation is accompanied by oxidation of ring-conjugated olefins in the lignin, while LPMO-catalyzed oxidation of phenolic hydroxyls leads to the required priming reduction of the enzyme. The discovery that light-driven abiotic reactions in Nature can fuel H2O2-dependent redox enzymes involved in deconstructing lignocellulose may offer opportunities for bioprocessing and provides an enzymatic explanation for the known effect of visible light on biomass conversion.

Suggested Citation

  • Eirik G. Kommedal & Camilla F. Angeltveit & Leesa J. Klau & Iván Ayuso-Fernández & Bjørnar Arstad & Simen G. Antonsen & Yngve Stenstrøm & Dag Ekeberg & Francisco Gírio & Florbela Carvalheiro & Svein J, 2023. "Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36660-4
    DOI: 10.1038/s41467-023-36660-4
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    References listed on IDEAS

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    1. Alex Kirui & Wancheng Zhao & Fabien Deligey & Hui Yang & Xue Kang & Frederic Mentink-Vigier & Tuo Wang, 2022. "Carbohydrate-aromatic interface and molecular architecture of lignocellulose," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Amy T. Austin & Lucía Vivanco, 2006. "Plant litter decomposition in a semi-arid ecosystem controlled by photodegradation," Nature, Nature, vol. 442(7102), pages 555-558, August.
    3. D. Cannella & K. B. Möllers & N.-U. Frigaard & P. E. Jensen & M. J. Bjerrum & K. S. Johansen & C. Felby, 2016. "Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme," Nature Communications, Nature, vol. 7(1), pages 1-8, September.
    4. Bastien Bissaro & Eirik Kommedal & Åsmund K. Røhr & Vincent G. H. Eijsink, 2020. "Controlled depolymerization of cellulose by light-driven lytic polysaccharide oxygenases," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
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