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Engraftment of allogeneic iPS cell-derived cartilage organoid in a primate model of articular cartilage defect

Author

Listed:
  • Kengo Abe

    (Osaka University
    Kyoto University
    Kyoto University)

  • Akihiro Yamashita

    (Osaka University
    Kyoto University)

  • Miho Morioka

    (Osaka University)

  • Nanao Horike

    (Osaka University
    Kyoto University)

  • Yoshiaki Takei

    (Kyoto University
    Regenerative Medicine Technology Department, Healthcare R&D Center, Asahi Kasei Corporation)

  • Saeko Koyamatsu

    (Osaka University)

  • Keisuke Okita

    (Kyoto University)

  • Shuichi Matsuda

    (Kyoto University)

  • Noriyuki Tsumaki

    (Osaka University
    Kyoto University
    Osaka University)

Abstract

Induced pluripotent stem cells (iPSCs) are a promising resource for allogeneic cartilage transplantation to treat articular cartilage defects that do not heal spontaneously and often progress to debilitating conditions, such as osteoarthritis. However, to the best of our knowledge, allogeneic cartilage transplantation into primate models has never been assessed. Here, we show that allogeneic iPSC-derived cartilage organoids survive and integrate as well as are remodeled as articular cartilage in a primate model of chondral defects in the knee joints. Histological analysis revealed that allogeneic iPSC-derived cartilage organoids in chondral defects elicited no immune reaction and directly contributed to tissue repair for at least four months. iPSC-derived cartilage organoids integrated with the host native articular cartilage and prevented degeneration of the surrounding cartilage. Single-cell RNA-sequence analysis indicated that iPSC-derived cartilage organoids differentiated after transplantation, acquiring expression of PRG4 crucial for joint lubrication. Pathway analysis suggested the involvement of SIK3 inactivation. Our study outcomes suggest that allogeneic transplantation of iPSC-derived cartilage organoids may be clinically applicable for the treatment of patients with chondral defects of the articular cartilage; however further assessment of functional recovery long term after load bearing injuries is required.

Suggested Citation

  • Kengo Abe & Akihiro Yamashita & Miho Morioka & Nanao Horike & Yoshiaki Takei & Saeko Koyamatsu & Keisuke Okita & Shuichi Matsuda & Noriyuki Tsumaki, 2023. "Engraftment of allogeneic iPS cell-derived cartilage organoid in a primate model of articular cartilage defect," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36408-0
    DOI: 10.1038/s41467-023-36408-0
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    References listed on IDEAS

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    1. Gioele La Manno & Ruslan Soldatov & Amit Zeisel & Emelie Braun & Hannah Hochgerner & Viktor Petukhov & Katja Lidschreiber & Maria E. Kastriti & Peter Lönnerberg & Alessandro Furlan & Jean Fan & Lars E, 2018. "RNA velocity of single cells," Nature, Nature, vol. 560(7719), pages 494-498, August.
    2. Asuka Morizane & Tetsuhiro Kikuchi & Takuya Hayashi & Hiroshi Mizuma & Sayuki Takara & Hisashi Doi & Aya Mawatari & Matthew F. Glasser & Takashi Shiina & Hirohito Ishigaki & Yasushi Itoh & Keisuke Oki, 2017. "MHC matching improves engraftment of iPSC-derived neurons in non-human primates," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
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