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Rad52’s DNA annealing activity drives template switching associated with restarted DNA replication

Author

Listed:
  • Anastasiya Kishkevich

    (University of Oxford)

  • Sanjeeta Tamang

    (University of Oxford)

  • Michael O. Nguyen

    (University of Oxford)

  • Judith Oehler

    (University of Oxford)

  • Elena Bulmaga

    (University of Oxford)

  • Christos Andreadis

    (University of Oxford)

  • Carl A. Morrow

    (University of Oxford)

  • Manisha Jalan

    (University of Oxford)

  • Fekret Osman

    (University of Oxford)

  • Matthew C. Whitby

    (University of Oxford)

Abstract

It is thought that many of the simple and complex genomic rearrangements associated with congenital diseases and cancers stem from mistakes made during the restart of collapsed replication forks by recombination enzymes. It is hypothesised that this recombination-mediated restart process transitions from a relatively accurate initiation phase to a less accurate elongation phase characterised by extensive template switching between homologous, homeologous and microhomologous DNA sequences. Using an experimental system in fission yeast, where fork collapse is triggered by a site-specific replication barrier, we show that ectopic recombination, associated with the initiation of recombination-dependent replication (RDR), is driven mainly by the Rad51 recombinase, whereas template switching, during the elongation phase of RDR, relies more on DNA annealing by Rad52. This finding provides both evidence and a mechanistic basis for the transition hypothesis.

Suggested Citation

  • Anastasiya Kishkevich & Sanjeeta Tamang & Michael O. Nguyen & Judith Oehler & Elena Bulmaga & Christos Andreadis & Carl A. Morrow & Manisha Jalan & Fekret Osman & Matthew C. Whitby, 2022. "Rad52’s DNA annealing activity drives template switching associated with restarted DNA replication," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35060-4
    DOI: 10.1038/s41467-022-35060-4
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    References listed on IDEAS

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    Cited by:

    1. Judith Oehler & Carl A. Morrow & Matthew C. Whitby, 2023. "Gene duplication and deletion caused by over-replication at a fork barrier," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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