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Self-assembling nanofibrous bacteriophage microgels as sprayable antimicrobials targeting multidrug-resistant bacteria

Author

Listed:
  • Lei Tian

    (McMaster University)

  • Leon He

    (McMaster University)

  • Kyle Jackson

    (McMaster University)

  • Ahmed Saif

    (McMaster University)

  • Shadman Khan

    (McMaster University)

  • Zeqi Wan

    (McMaster University)

  • Tohid F. Didar

    (McMaster University
    McMaster University
    McMaster University)

  • Zeinab Hosseinidoust

    (McMaster University
    McMaster University
    McMaster University)

Abstract

Nanofilamentous bacteriophages (bacterial viruses) are biofunctional, self-propagating, and monodisperse natural building blocks for virus-built materials. Minifying phage-built materials to microscale offers the promise of expanding the range function for these biomaterials to sprays and colloidal bioassays/biosensors. Here, we crosslink half a million self-organized phages as the sole structural component to construct each soft microgel. Through an in-house developed, biologics-friendly, high-throughput template method, over 35,000 phage-built microgels are produced from every square centimetre of a peelable microporous film template, constituting a 13-billion phage community. The phage-exclusive microgels exhibit a self-organized, highly-aligned nanofibrous texture and tunable auto-fluorescence. Further preservation of antimicrobial activity was achieved by making hybrid protein-phage microgels. When loaded with potent virulent phages, these microgels effectively reduce heavy loads of multidrug-resistant Escherichia coli O157:H7 on food products, leading to up to 6 logs reduction in 9 hours and rendering food contaminant free.

Suggested Citation

  • Lei Tian & Leon He & Kyle Jackson & Ahmed Saif & Shadman Khan & Zeqi Wan & Tohid F. Didar & Zeinab Hosseinidoust, 2022. "Self-assembling nanofibrous bacteriophage microgels as sprayable antimicrobials targeting multidrug-resistant bacteria," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34803-7
    DOI: 10.1038/s41467-022-34803-7
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    References listed on IDEAS

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    1. Bryan B. Hsu & Isaac N. Plant & Lorena Lyon & Frances M. Anastassacos & Jeffrey C. Way & Pamela A. Silver, 2020. "In situ reprogramming of gut bacteria by oral delivery," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    2. Ran Meng & Mengqiu Jiang & Zhicheng Cui & Jeng-Yih Chang & Kailu Yang & Joanita Jakana & Xinzhe Yu & Zhao Wang & Bo Hu & Junjie Zhang, 2019. "Structural basis for the adsorption of a single-stranded RNA bacteriophage," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    3. Woo-Jae Chung & Jin-Woo Oh & Kyungwon Kwak & Byung Yang Lee & Joel Meyer & Eddie Wang & Alexander Hexemer & Seung-Wuk Lee, 2011. "Biomimetic self-templating supramolecular structures," Nature, Nature, vol. 478(7369), pages 364-368, October.
    4. Jin-Woo Oh & Woo-Jae Chung & Kwang Heo & Hyo-Eon Jin & Byung Yang Lee & Eddie Wang & Chris Zueger & Winnie Wong & Joel Meyer & Chuntae Kim & So-Young Lee & Won-Geun Kim & Marcin Zemla & Manfred Auer &, 2014. "Biomimetic virus-based colourimetric sensors," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
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    Cited by:

    1. Yintao Li & Longlong Fan & Xinyan Xu & Yang Sun & Wei Wang & Bin Li & Samuel S. Veroneau & Pengfei Ji, 2024. "Hierarchical organic microspheres from diverse molecular building blocks," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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