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Structural basis for the adsorption of a single-stranded RNA bacteriophage

Author

Listed:
  • Ran Meng

    (Texas A&M University)

  • Mengqiu Jiang

    (Texas A&M University)

  • Zhicheng Cui

    (Texas A&M University)

  • Jeng-Yih Chang

    (Texas A&M University)

  • Kailu Yang

    (Texas A&M University
    Stanford University)

  • Joanita Jakana

    (Baylor College of Medicine)

  • Xinzhe Yu

    (Baylor College of Medicine)

  • Zhao Wang

    (Baylor College of Medicine)

  • Bo Hu

    (The University of Texas Health Science Center at Houston)

  • Junjie Zhang

    (Texas A&M University)

Abstract

Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.

Suggested Citation

  • Ran Meng & Mengqiu Jiang & Zhicheng Cui & Jeng-Yih Chang & Kailu Yang & Joanita Jakana & Xinzhe Yu & Zhao Wang & Bo Hu & Junjie Zhang, 2019. "Structural basis for the adsorption of a single-stranded RNA bacteriophage," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11126-8
    DOI: 10.1038/s41467-019-11126-8
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    Cited by:

    1. Ran Meng & Zhongliang Xing & Jeng-Yih Chang & Zihao Yu & Jirapat Thongchol & Wen Xiao & Yuhang Wang & Karthik Chamakura & Zhiqi Zeng & Fengbin Wang & Ry Young & Lanying Zeng & Junjie Zhang, 2024. "Structural basis of Acinetobacter type IV pili targeting by an RNA virus," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    2. Lei Tian & Leon He & Kyle Jackson & Ahmed Saif & Shadman Khan & Zeqi Wan & Tohid F. Didar & Zeinab Hosseinidoust, 2022. "Self-assembling nanofibrous bacteriophage microgels as sprayable antimicrobials targeting multidrug-resistant bacteria," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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