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Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

Author

Listed:
  • Muxiong Chen

    (Zhejiang University School of Medicine
    Zhejiang University Medical Center)

  • Zhe Feng

    (Zhejiang University)

  • Xiaoxiao Fan

    (Zhejiang University)

  • Jun Sun

    (Zhejiang University School of Medicine)

  • Weihang Geng

    (Zhejiang University)

  • Tianxiang Wu

    (Zhejiang University)

  • Jinghao Sheng

    (Zhejiang University School of Medicine
    Zhejiang University Medical Center)

  • Jun Qian

    (Zhejiang University)

  • Zhengping Xu

    (Zhejiang University School of Medicine
    Zhejiang University Medical Center)

Abstract

High spatial resolution, low background, and deep tissue penetration have made near-infrared II (NIR-II) fluorescence imaging one of the most critical tools for in vivo observation and measurement. However, the relatively short retention time and potential toxicity of synthetic NIR-II fluorophores limit their long-term application. Here, we report the use of infrared fluorescent proteins (iRFPs) as in vitro and in vivo NIR-II probes permitting prolonged continuous imaging (up to 15 months). As a representative example, iRFP713 is knocked into the mouse genome to generate a transgenic model to allow temporal and/or spatial expression control of the probe. To demonstrate its feasibility in a genuine diagnostic context, we adopt two liver regeneration models and successfully track the process for a week. The performance and monitoring efficacy are comparable to those of μCT and superior to those of indocyanine green dye. We are also able to effectively observe the pancreas, despite its deep location, under both physiological and pathological conditions. These results indicate that the iRFP-assisted NIR-II fluorescence system is suitable for monitoring various tissues and in vivo biological processes, providing a powerful noninvasive long-term imaging platform.

Suggested Citation

  • Muxiong Chen & Zhe Feng & Xiaoxiao Fan & Jun Sun & Weihang Geng & Tianxiang Wu & Jinghao Sheng & Jun Qian & Zhengping Xu, 2022. "Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34274-w
    DOI: 10.1038/s41467-022-34274-w
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    1. Luyan Wu & Yusuke Ishigaki & Wenhui Zeng & Takashi Harimoto & Baoli Yin & Yinghan Chen & Shiyi Liao & Yongchun Liu & Yidan Sun & Xiaobo Zhang & Ying Liu & Yong Liang & Pengfei Sun & Takanori Suzuki & , 2021. "Generation of hydroxyl radical-activatable ratiometric near-infrared bimodal probes for early monitoring of tumor response to therapy," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    2. Wenting Yang & Yuandong Tao & Yan Wu & Xinyuan Zhao & Weijie Ye & Dianyuan Zhao & Ling Fu & Caiping Tian & Jing Yang & Fuchu He & Li Tang, 2019. "Neutrophils promote the development of reparative macrophages mediated by ROS to orchestrate liver repair," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
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