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Flexible hyperspectral surface plasmon resonance microscopy

Author

Listed:
  • Ziwei Liu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jingning Wu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Chen Cai

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Bo Yang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhi-mei Qi

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Optical techniques for visualization and quantification of chemical and biological analytes are always highly desirable. Here we show a hyperspectral surface plasmon resonance microscopy (HSPRM) system that uses a hyperspectral microscope to analyze the selected area of SPR image produced by a prism-based spectral SPR sensor. The HSPRM system enables monochromatic and polychromatic SPR imaging and single-pixel spectral SPR sensing, as well as two-dimensional quantification of thin films with the measured resonance-wavelength images. We performed pixel-by-pixel calibration of the incident angle to remove pixel-to-pixel differences in SPR sensitivity, and demonstrated the HSPRM’s capabilities by using it to quantify monolayer graphene thickness distribution, inhomogeneous protein adsorption and single-cell adhesion. The HSPRM system has a wide spectral range from 400 nm to 1000 nm, an optional field of view from 0.884 mm2 to 0.003 mm2 and a high lateral resolution of 1.2 μm, demonstrating an innovative breakthrough in SPR sensor technology.

Suggested Citation

  • Ziwei Liu & Jingning Wu & Chen Cai & Bo Yang & Zhi-mei Qi, 2022. "Flexible hyperspectral surface plasmon resonance microscopy," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34196-7
    DOI: 10.1038/s41467-022-34196-7
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    References listed on IDEAS

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    1. Guangzhong Ma & Runli Liang & Zijian Wan & Shaopeng Wang, 2021. "Critical angle reflection imaging for quantification of molecular interactions on glass surface," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    2. Iban Amenabar & Simon Poly & Monika Goikoetxea & Wiwat Nuansing & Peter Lasch & Rainer Hillenbrand, 2017. "Hyperspectral infrared nanoimaging of organic samples based on Fourier transform infrared nanospectroscopy," Nature Communications, Nature, vol. 8(1), pages 1-10, April.
    3. Liang-Jin Xu & Xinsong Lin & Qingquan He & Michael Worku & Biwu Ma, 2020. "Highly efficient eco-friendly X-ray scintillators based on an organic manganese halide," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
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