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Telomere-to-telomere genome sequence of the model mould pathogen Aspergillus fumigatus

Author

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  • Paul Bowyer

    (University of Manchester)

  • Andrew Currin

    (The University of Manchester)

  • Daniela Delneri

    (The University of Manchester)

  • Marcin G. Fraczek

    (The University of Manchester)

Abstract

The pathogenic fungus Aspergillus fumigatus is a major etiological agent of fungal invasive and chronic diseases affecting tens of millions of individuals worldwide. Draft genome sequences of two clinical isolates (Af293 and A1163) are commonly used as reference genomes for analyses of clinical and environmental strains. However, the reference sequences lack coverage of centromeres, an accurate sequence for ribosomal repeats, and a comprehensive annotation of chromosomal rearrangements such as translocations and inversions. Here, we used PacBio Single Molecule Real-Time (SMRT), Oxford Nanopore and Illumina HiSeq sequencing for de novo genome assembly and polishing of two laboratory reference strains of A. fumigatus, CEA10 (parental isolate of A1163) and its descendant A1160. We generated full length chromosome assemblies and a comprehensive telomere-to-telomere coverage for CEA10 and near complete assembly of A1160 including ribosomal repeats and the sequences of centromeres, which we discovered to be composed of long transposon elements. We envision these high-quality reference genomes will become fundamental resources to study A. fumigatus biology, pathogenicity and virulence, and to discover more effective treatments against diseases caused by this fungus.

Suggested Citation

  • Paul Bowyer & Andrew Currin & Daniela Delneri & Marcin G. Fraczek, 2022. "Telomere-to-telomere genome sequence of the model mould pathogen Aspergillus fumigatus," Nature Communications, Nature, vol. 13(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32924-7
    DOI: 10.1038/s41467-022-32924-7
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    References listed on IDEAS

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    1. Takanori Furukawa & Norman van Rhijn & Marcin Fraczek & Fabio Gsaller & Emma Davies & Paul Carr & Sara Gago & Rachael Fortune-Grant & Sayema Rahman & Jane Mabey Gilsenan & Emma Houlder & Caitlin H. Ko, 2020. "The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
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