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VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC

Author

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  • Pawel Mikulski

    (John Innes Centre
    University of Oxford)

  • Philip Wolff

    (John Innes Centre)

  • Tiancong Lu

    (John Innes Centre
    Chinese Academy of Sciences)

  • Mathias Nielsen

    (John Innes Centre)

  • Elsa Franco Echevarria

    (MRC Laboratory of Molecular Biology)

  • Danling Zhu

    (John Innes Centre
    Southern University of Science and Technology)

  • Julia I. Questa

    (John Innes Centre
    Centre for Research in Agricultural Genomics)

  • Gerhard Saalbach

    (Biological Chemistry, John Innes Centre)

  • Carlo Martins

    (Biological Chemistry, John Innes Centre)

  • Caroline Dean

    (John Innes Centre
    MRC Laboratory of Molecular Biology)

Abstract

Polycomb (PcG) silencing is crucial for development, but how targets are specified remains incompletely understood. The cold-induced Polycomb Repressive Complex 2 (PRC2) silencing of Arabidopsis thaliana FLOWERING LOCUS C (FLC) provides an excellent system to elucidate PcG regulation. Association of the DNA binding protein VAL1 to FLC PcG nucleation regionis an important step. VAL1 co-immunoprecipitates APOPTOSIS AND SPLICING ASSOCIATED PROTEIN (ASAP) complex and PRC1. Here, we show that ASAP and PRC1 are necessary for co-transcriptional repression and chromatin regulation at FLC. ASAP mutants affect FLC transcription in warm conditions, but the rate of FLC silencing in the cold is unaffected. PRC1-mediated H2Aub accumulation increases at the FLC nucleation region during cold, but unlike the PRC2-delivered H3K27me3, does not spread across the locus. H2Aub thus involved in the transition to epigenetic silencing at FLC, facilitating H3K27me3 accumulation and long-term epigenetic memory. Overall, our work highlights the importance of VAL1 as an assembly platform co-ordinating activities necessary for epigenetic silencing at FLC.

Suggested Citation

  • Pawel Mikulski & Philip Wolff & Tiancong Lu & Mathias Nielsen & Elsa Franco Echevarria & Danling Zhu & Julia I. Questa & Gerhard Saalbach & Carlo Martins & Caroline Dean, 2022. "VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32897-7
    DOI: 10.1038/s41467-022-32897-7
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    References listed on IDEAS

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    1. Xiaochang Yin & Francisco J. Romero-Campero & Pedro de Los Reyes & Peng Yan & Jing Yang & Guangmei Tian & XiaoZeng Yang & Xiaorong Mo & Shuangshuang Zhao & Myriam Calonje & Yue Zhou, 2021. "H2AK121ub in Arabidopsis associates with a less accessible chromatin state at transcriptional regulation hotspots," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Pan Zhu & Clare Lister & Caroline Dean, 2021. "Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression," Nature, Nature, vol. 599(7886), pages 657-661, November.
    3. Congyao Xu & Zhe Wu & Hong-Chao Duan & Xiaofeng Fang & Guifang Jia & Caroline Dean, 2021. "R-loop resolution promotes co-transcriptional chromatin silencing," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    4. Yang Bi & Zhiping Deng & Weimin Ni & Ruben Shrestha & Dasha Savage & Thomas Hartwig & Sunita Patil & Su Hyun Hong & Zhenzhen Zhang & Juan A. Oses-Prieto & Kathy H. Li & Peter H. Quail & Alma L. Burlin, 2021. "Arabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    5. Szymon Swiezewski & Fuquan Liu & Andreas Magusin & Caroline Dean, 2009. "Cold-induced silencing by long antisense transcripts of an Arabidopsis Polycomb target," Nature, Nature, vol. 462(7274), pages 799-802, December.
    6. Haithem Barbour & Salima Daou & Michael Hendzel & El Bachir Affar, 2020. "Polycomb group-mediated histone H2A monoubiquitination in epigenome regulation and nuclear processes," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
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