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SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings

Author

Listed:
  • Mitchell D. Ramuta

    (University of Wisconsin-Madison)

  • Christina M. Newman

    (University of Wisconsin-Madison)

  • Savannah F. Brakefield

    (University of Wisconsin-Madison)

  • Miranda R. Stauss

    (Wisconsin National Primate Research Center)

  • Roger W. Wiseman

    (University of Wisconsin-Madison
    Wisconsin National Primate Research Center)

  • Amanda Kita-Yarbro

    (Public Health Madison & Dane County)

  • Eli J. O’Connor

    (EAGLE School)

  • Neeti Dahal

    (Wisconsin Veterinary Diagnostic Laboratory)

  • Ailam Lim

    (Wisconsin Veterinary Diagnostic Laboratory)

  • Keith P. Poulsen

    (Wisconsin Veterinary Diagnostic Laboratory)

  • Nasia Safdar

    (University of Wisconsin Hospitals and Clinics)

  • John A. Marx

    (University of Wisconsin Hospitals and Clinics)

  • Molly A. Accola

    (University of Wisconsin Hospitals and Clinics)

  • William M. Rehrauer

    (University of Wisconsin-Madison
    University of Wisconsin Hospitals and Clinics)

  • Julia A. Zimmer

    (City of Milwaukee Health Department Laboratory)

  • Manjeet Khubbar

    (City of Milwaukee Health Department Laboratory)

  • Lucas J. Beversdorf

    (City of Milwaukee Health Department Laboratory)

  • Emma C. Boehm

    (University of Minnesota, Twin Cities)

  • David Castañeda

    (University of Minnesota, Twin Cities)

  • Clayton Rushford

    (University of Missouri, School of Medicine)

  • Devon A. Gregory

    (University of Missouri, School of Medicine)

  • Joseph D. Yao

    (Mayo Clinic)

  • Sanjib Bhattacharyya

    (City of Milwaukee Health Department Laboratory)

  • Marc C. Johnson

    (University of Missouri, School of Medicine)

  • Matthew T. Aliota

    (University of Minnesota, Twin Cities)

  • Thomas C. Friedrich

    (University of Wisconsin-Madison)

  • David H. O’Connor

    (University of Wisconsin-Madison
    Wisconsin National Primate Research Center)

  • Shelby L. O’Connor

    (University of Wisconsin-Madison
    Wisconsin National Primate Research Center)

Abstract

Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess the risk of transmission in congregate spaces. We deployed active air samplers to monitor the presence of SARS-CoV-2 in real-world settings across communities in the Upper Midwestern states of Wisconsin and Minnesota. Over 29 weeks, we collected 527 air samples from 15 congregate settings. We detected 106 samples that were positive for SARS-CoV-2 viral RNA, demonstrating that SARS-CoV-2 can be detected in continuous air samples collected from a variety of real-world settings. We expanded the utility of air surveillance to test for 40 other respiratory pathogens. Surveillance data revealed differences in timing and location of SARS-CoV-2 and influenza A virus detection. In addition, we obtained SARS-CoV-2 genome sequences from air samples to identify variant lineages. Collectively, this shows air sampling is a scalable, high throughput surveillance tool that could be used in conjunction with other methods for detecting respiratory pathogens in congregate settings.

Suggested Citation

  • Mitchell D. Ramuta & Christina M. Newman & Savannah F. Brakefield & Miranda R. Stauss & Roger W. Wiseman & Amanda Kita-Yarbro & Eli J. O’Connor & Neeti Dahal & Ailam Lim & Keith P. Poulsen & Nasia Saf, 2022. "SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32406-w
    DOI: 10.1038/s41467-022-32406-w
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    References listed on IDEAS

    as
    1. Kevin Credit, 2020. "Neighbourhood inequity: Exploring the factors underlying racial and ethnic disparities in COVID‐19 testing and infection rates using ZIP code data in Chicago and New York," Regional Science Policy & Practice, Wiley Blackwell, vol. 12(6), pages 1249-1271, December.
    2. Davida S. Smyth & Monica Trujillo & Devon A. Gregory & Kristen Cheung & Anna Gao & Maddie Graham & Yue Guan & Caitlyn Guldenpfennig & Irene Hoxie & Sherin Kannoly & Nanami Kubota & Terri D. Lyddon & M, 2022. "Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    3. Sasha Harris-Lovett & Kara L. Nelson & Paloma Beamer & Heather N. Bischel & Aaron Bivins & Andrea Bruder & Caitlyn Butler & Todd D. Camenisch & Susan K. De Long & Smruthi Karthikeyan & David A. Larsen, 2021. "Wastewater Surveillance for SARS-CoV-2 on College Campuses: Initial Efforts, Lessons Learned, and Research Needs," IJERPH, MDPI, vol. 18(9), pages 1-20, April.
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    1. Bao Li & Baobao Lin & Yan Wang & Ye Shi & Wu Zeng & Yulan Zhao & Yin Gu & Chang Liu & Hui Gao & Hao Cheng & Xiaoqun Zheng & Guangxin Xiang & Guiqiang Wang & Peng Liu, 2024. "Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Sung Hee Ko & Pierce Radecki & Frida Belinky & Jinal N. Bhiman & Susan Meiring & Jackie Kleynhans & Daniel Amoako & Vanessa Guerra Canedo & Margaret Lucas & Dikeledi Kekana & Neil Martinson & Limakats, 2024. "Rapid intra-host diversification and evolution of SARS-CoV-2 in advanced HIV infection," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Joren Raymenants & Caspar Geenen & Lore Budts & Jonathan Thibaut & Marijn Thijssen & Hannelore Mulder & Sarah Gorissen & Bastiaan Craessaerts & Lies Laenen & Kurt Beuselinck & Sien Ombelet & Els Keyae, 2023. "Indoor air surveillance and factors associated with respiratory pathogen detection in community settings in Belgium," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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