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Real-time precision opto-control of chemical processes in live cells

Author

Listed:
  • Matthew G. Clark

    (Purdue University)

  • Gil A. Gonzalez

    (Purdue University)

  • Yiyang Luo

    (Purdue University)

  • Jesus A. Aldana-Mendoza

    (Purdue University)

  • Mark S. Carlsen

    (Purdue University)

  • Gregory Eakins

    (Purdue University)

  • Mingji Dai

    (Purdue University
    Purdue Center for Cancer Research)

  • Chi Zhang

    (Purdue University
    Purdue Center for Cancer Research
    Purdue Institute of Inflammation, Immunology, and Infectious Disease)

Abstract

Precision control of molecular activities and chemical reactions in live cells is a long-sought capability by life scientists. No existing technology can probe molecular targets in cells and simultaneously control the activities of only these targets at high spatial precision. We develop a real-time precision opto-control (RPOC) technology that detects a chemical-specific optical response from molecular targets during laser scanning and uses the optical signal to couple a separate laser to only interact with these molecules without affecting other sample locations. We demonstrate precision control of molecular states of a photochromic molecule in different regions of the cells. We also synthesize a photoswitchable compound and use it with RPOC to achieve site-specific inhibition of microtubule polymerization and control of organelle dynamics in live cells. RPOC can automatically detect and control biomolecular activities and chemical processes in dynamic living samples with submicron spatial accuracy, fast response time, and high chemical specificity.

Suggested Citation

  • Matthew G. Clark & Gil A. Gonzalez & Yiyang Luo & Jesus A. Aldana-Mendoza & Mark S. Carlsen & Gregory Eakins & Mingji Dai & Chi Zhang, 2022. "Real-time precision opto-control of chemical processes in live cells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32071-z
    DOI: 10.1038/s41467-022-32071-z
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    References listed on IDEAS

    as
    1. Jianpeng Ao & Xiaofeng Fang & Xianchong Miao & Jiwei Ling & Hyunchul Kang & Sungnam Park & Changfeng Wu & Minbiao Ji, 2021. "Switchable stimulated Raman scattering microscopy with photochromic vibrational probes," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Antoine R. Adamantidis & Feng Zhang & Alexander M. Aravanis & Karl Deisseroth & Luis de Lecea, 2007. "Neural substrates of awakening probed with optogenetic control of hypocretin neurons," Nature, Nature, vol. 450(7168), pages 420-424, November.
    Full references (including those not matched with items on IDEAS)

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