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Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

Author

Listed:
  • Jianpeng Ao

    (Fudan University)

  • Xiaofeng Fang

    (Southern University of Science and Technology)

  • Xianchong Miao

    (Fudan University)

  • Jiwei Ling

    (Fudan University)

  • Hyunchul Kang

    (Korea University)

  • Sungnam Park

    (Korea University)

  • Changfeng Wu

    (Southern University of Science and Technology)

  • Minbiao Ji

    (Fudan University)

Abstract

Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding “on” or “off” SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.

Suggested Citation

  • Jianpeng Ao & Xiaofeng Fang & Xianchong Miao & Jiwei Ling & Hyunchul Kang & Sungnam Park & Changfeng Wu & Minbiao Ji, 2021. "Switchable stimulated Raman scattering microscopy with photochromic vibrational probes," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23407-2
    DOI: 10.1038/s41467-021-23407-2
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    Cited by:

    1. Yueli Yang & Xueyang Bai & Fanghao Hu, 2024. "Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Matthew G. Clark & Gil A. Gonzalez & Yiyang Luo & Jesus A. Aldana-Mendoza & Mark S. Carlsen & Gregory Eakins & Mingji Dai & Chi Zhang, 2022. "Real-time precision opto-control of chemical processes in live cells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Zhijie Liu & Wei Su & Jianpeng Ao & Min Wang & Qiuli Jiang & Jie He & Hua Gao & Shu Lei & Jinshan Nie & Xuefeng Yan & Xiaojing Guo & Pinghong Zhou & Hao Hu & Minbiao Ji, 2022. "Instant diagnosis of gastroscopic biopsy via deep-learned single-shot femtosecond stimulated Raman histology," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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