IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-31996-9.html
   My bibliography  Save this article

CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation

Author

Listed:
  • Jian Huang

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University)

  • Luxin Wang

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University)

  • Yunli Shen

    (Tongji University School of Medicine
    Tongji University School of Medicine)

  • Shengqi Zhang

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University)

  • Yaqun Zhou

    (Tongji University School of Medicine)

  • Jimin Du

    (Tongji University School of Medicine)

  • Xiue Ma

    (Tongji University School of Medicine
    Tongji University)

  • Yi Liu

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University)

  • Dandan Liang

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University)

  • Dan Shi

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University)

  • Honghui Ma

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University
    Chinese Academy of Medical Sciences)

  • Li Li

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University
    Chinese Academy of Medical Sciences)

  • Qi Zhang

    (Tongji University School of Medicine
    Tongji University School of Medicine)

  • Yi-Han Chen

    (Tongji University School of Medicine
    Tongji University School of Medicine
    Tongji University
    Tongji University School of Medicine)

Abstract

Kinase-catalyzed phosphorylation plays a crucial role in pathological cardiac hypertrophy. Here, we show that CDC-like kinase 4 (CLK4) is a critical regulator of cardiomyocyte hypertrophy and heart failure. Knockdown of Clk4 leads to pathological cardiomyocyte hypertrophy, while overexpression of Clk4 confers resistance to phenylephrine-induced cardiomyocyte hypertrophy. Cardiac-specific Clk4-knockout mice manifest pathological myocardial hypertrophy with progressive left ventricular systolic dysfunction and heart dilation. Further investigation identifies nexilin (NEXN) as the direct substrate of CLK4, and overexpression of a phosphorylation-mimic mutant of NEXN is sufficient to reverse the hypertrophic growth of cardiomyocytes induced by Clk4 knockdown. Importantly, restoring phosphorylation of NEXN ameliorates myocardial hypertrophy in mice with cardiac-specific Clk4 deletion. We conclude that CLK4 regulates cardiac function through phosphorylation of NEXN, and its deficiency may lead to pathological cardiac hypertrophy. CLK4 is a potential intervention target for the prevention and treatment of heart failure.

Suggested Citation

  • Jian Huang & Luxin Wang & Yunli Shen & Shengqi Zhang & Yaqun Zhou & Jimin Du & Xiue Ma & Yi Liu & Dandan Liang & Dan Shi & Honghui Ma & Li Li & Qi Zhang & Yi-Han Chen, 2022. "CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31996-9
    DOI: 10.1038/s41467-022-31996-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-31996-9
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-022-31996-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Eleni Petsalaki & George Zachos, 2016. "Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint," Nature Communications, Nature, vol. 7(1), pages 1-13, September.
    2. Alexander Karlas & Nikolaus Machuy & Yujin Shin & Klaus-Peter Pleissner & Anita Artarini & Dagmar Heuer & Daniel Becker & Hany Khalil & Lesley A. Ogilvie & Simone Hess & André P. Mäurer & Elke Müller , 2010. "Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication," Nature, Nature, vol. 463(7282), pages 818-822, February.
    3. Jun Li & Changming Li & Dasheng Zhang & Dan Shi & Man Qi & Jing Feng & Tianyou Yuan & Xinran Xu & Dandan Liang & Liang Xu & Hong Zhang & Yi Liu & Jinjin Chen & Jiangchuan Ye & Weifang Jiang & Yingyu C, 2014. "SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain," Nature Communications, Nature, vol. 5(1), pages 1-13, December.
    4. Hannah V. Woodcock & Jessica D. Eley & Delphine Guillotin & Manuela Platé & Carmel B. Nanthakumar & Matteo Martufi & Simon Peace & Gerard Joberty & Daniel Poeckel & Robert B. Good & Adam R. Taylor & N, 2019. "The mTORC1/4E-BP1 axis represents a critical signaling node during fibrogenesis," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Valentina Gandin & Brian P. English & Melanie Freeman & Louis-Philippe Leroux & Stephan Preibisch & Deepika Walpita & Maritza Jaramillo & Robert H. Singer, 2022. "Cap-dependent translation initiation monitored in living cells," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Joseph D. Trimarco & Sarah L. Nelson & Ryan R. Chaparian & Alexandra I. Wells & Nathan B. Murray & Parastoo Azadi & Carolyn B. Coyne & Nicholas S. Heaton, 2022. "Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31996-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.