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Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication

Author

Listed:
  • Alexander Karlas

    (Molecular Biology Department,)

  • Nikolaus Machuy

    (Molecular Biology Department,)

  • Yujin Shin

    (Molecular Biology Department,)

  • Klaus-Peter Pleissner

    (Core Facility Bioinformatics, Max Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany)

  • Anita Artarini

    (Molecular Biology Department,)

  • Dagmar Heuer

    (Molecular Biology Department,)

  • Daniel Becker

    (Molecular Biology Department,)

  • Hany Khalil

    (Molecular Biology Department,)

  • Lesley A. Ogilvie

    (Molecular Biology Department,)

  • Simone Hess

    (Molecular Biology Department,
    Present addresses: Hannover Medical School, 30625 Hannover, Germany (S.H.); Exploratory Research Space, RWTH Aachen University, Templergraben 55, 52056 Aachen, Germany (E.M.); Department of Microbiology, Biocenter, University of Würzburg, 97074 Würzburg, Germany (T.R.).)

  • André P. Mäurer

    (Molecular Biology Department,)

  • Elke Müller

    (Molecular Biology Department,
    Present addresses: Hannover Medical School, 30625 Hannover, Germany (S.H.); Exploratory Research Space, RWTH Aachen University, Templergraben 55, 52056 Aachen, Germany (E.M.); Department of Microbiology, Biocenter, University of Würzburg, 97074 Würzburg, Germany (T.R.).)

  • Thorsten Wolff

    (Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany)

  • Thomas Rudel

    (Molecular Biology Department,
    Present addresses: Hannover Medical School, 30625 Hannover, Germany (S.H.); Exploratory Research Space, RWTH Aachen University, Templergraben 55, 52056 Aachen, Germany (E.M.); Department of Microbiology, Biocenter, University of Würzburg, 97074 Würzburg, Germany (T.R.).)

  • Thomas F. Meyer

    (Molecular Biology Department,)

Abstract

Host factors in flu infectivity Two genome-wide RNA interference screens published in this issue identify human host factors required for influenza A virus replication in lung epithelia cell lines. König et al. identify 295 host genes required for influenza replication. Of those, 219 are required for efficient wild-type virus growth, and 23 are required for viral entry. Karlas et al. report the discovery of 287 host genes influencing virus replication. An independent assay confirmed 168 hits (59%) inhibiting either the endemic H1N1 (119 hits) or the current pandemic swine-origin (121 hits) influenza A virus strains, with an overlap of 60%. These studies should provide a number of potential targets for host factor-directed antivirals for treatment of influenza viral infection.

Suggested Citation

  • Alexander Karlas & Nikolaus Machuy & Yujin Shin & Klaus-Peter Pleissner & Anita Artarini & Dagmar Heuer & Daniel Becker & Hany Khalil & Lesley A. Ogilvie & Simone Hess & André P. Mäurer & Elke Müller , 2010. "Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication," Nature, Nature, vol. 463(7282), pages 818-822, February.
    • Handle: RePEc:nat:nature:v:463:y:2010:i:7282:d:10.1038_nature08760
    DOI: 10.1038/nature08760
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    Cited by:

    1. Jian Huang & Luxin Wang & Yunli Shen & Shengqi Zhang & Yaqun Zhou & Jimin Du & Xiue Ma & Yi Liu & Dandan Liang & Dan Shi & Honghui Ma & Li Li & Qi Zhang & Yi-Han Chen, 2022. "CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Joseph D. Trimarco & Sarah L. Nelson & Ryan R. Chaparian & Alexandra I. Wells & Nathan B. Murray & Parastoo Azadi & Carolyn B. Coyne & Nicholas S. Heaton, 2022. "Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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