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Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

Author

Listed:
  • Kenneth Danh

    (Enable Biosciences Inc)

  • Donna Grace Karp

    (Enable Biosciences Inc)

  • Malvika Singhal

    (Enable Biosciences Inc)

  • Akshaya Tankasala

    (Enable Biosciences Inc)

  • David Gebhart

    (Enable Biosciences Inc)

  • Felipe Jesus Cortez

    (Enable Biosciences Inc)

  • Devangkumar Tandel

    (Enable Biosciences Inc)

  • Peter V. Robinson

    (Enable Biosciences Inc)

  • David Seftel

    (Enable Biosciences Inc)

  • Mars Stone

    (Vitalant Research Institute)

  • Graham Simmons

    (Vitalant Research Institute)

  • Anil Bagri

    (Cerus Corporation)

  • Martin A. Schreiber

    (Oregon Health & Science University)

  • Andreas Buser

    (University Hospital Basel, University of Basel)

  • Andreas Holbro

    (University Hospital Basel, University of Basel)

  • Manuel Battegay

    (University Hospital Basel, University of Basel)

  • Mary Kate Morris

    (California Department of Public Health)

  • Carl Hanson

    (California Department of Public Health)

  • John R. Mills

    (Mayo Clinic)

  • Dane Granger

    (Mayo Clinic)

  • Elitza S. Theel

    (Mayo Clinic)

  • James R. Stubbs

    (Mayo Clinic)

  • Laurence M. Corash

    (Cerus Corporation)

  • Cheng-ting Tsai

    (Enable Biosciences Inc)

Abstract

An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91–97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.

Suggested Citation

  • Kenneth Danh & Donna Grace Karp & Malvika Singhal & Akshaya Tankasala & David Gebhart & Felipe Jesus Cortez & Devangkumar Tandel & Peter V. Robinson & David Seftel & Mars Stone & Graham Simmons & Anil, 2022. "Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31796-1
    DOI: 10.1038/s41467-022-31796-1
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    References listed on IDEAS

    as
    1. Fabian Schmidt & Yiska Weisblum & Magdalena Rutkowska & Daniel Poston & Justin DaSilva & Fengwen Zhang & Eva Bednarski & Alice Cho & Dennis J. Schaefer-Babajew & Christian Gaebler & Marina Caskey & Mi, 2021. "High genetic barrier to SARS-CoV-2 polyclonal neutralizing antibody escape," Nature, Nature, vol. 600(7889), pages 512-516, December.
    2. Stefania Dispinseri & Massimiliano Secchi & Maria Franca Pirillo & Monica Tolazzi & Martina Borghi & Cristina Brigatti & Maria Laura Angelis & Marco Baratella & Elena Bazzigaluppi & Giulietta Venturi , 2021. "Neutralizing antibody responses to SARS-CoV-2 in symptomatic COVID-19 is persistent and critical for survival," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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