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Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis

Author

Listed:
  • Hongliang Zhang

    (Ruhr University Bochum)

  • Julia Quintana

    (Ruhr University Bochum)

  • Koray Ütkür

    (University of Kassel)

  • Lorenz Adrian

    (Helmholtz Centre for Environmental Research—UFZ
    Technische Universität Berlin)

  • Harmen Hawer

    (University of Kassel)

  • Klaus Mayer

    (Roche Innovation Center Munich)

  • Xiaodi Gong

    (University of Heidelberg)

  • Leonardo Castanedo

    (Ruhr University Bochum)

  • Anna Schulten

    (Ruhr University Bochum)

  • Nadežda Janina

    (Ruhr University Bochum)

  • Marcus Peters

    (Ruhr University Bochum)

  • Markus Wirtz

    (University of Heidelberg)

  • Ulrich Brinkmann

    (Roche Innovation Center Munich)

  • Raffael Schaffrath

    (University of Kassel)

  • Ute Krämer

    (Ruhr University Bochum)

Abstract

Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target of diphtheria toxin. Diphthamide biosynthesis depends on the 4Fe-4S-cluster protein Dph1 catalyzing the first committed step, as well as Dph2 to Dph7, in yeast and mammals. Here we show that diphthamide modification of eEF2 is conserved in Arabidopsis thaliana and requires AtDPH1. Ribosomal −1 frameshifting-error rates are increased in Arabidopsis dph1 mutants, similar to yeast and mice. Compared to the wild type, shorter roots and smaller rosettes of dph1 mutants result from fewer formed cells. TARGET OF RAPAMYCIN (TOR) kinase activity is attenuated, and autophagy is activated, in dph1 mutants. Under abiotic stress diphthamide-unmodified eEF2 accumulates in wild-type seedlings, most strongly upon heavy metal excess, which is conserved in human cells. In summary, our results suggest that diphthamide contributes to the functionality of the translational machinery monitored by plants to regulate growth.

Suggested Citation

  • Hongliang Zhang & Julia Quintana & Koray Ütkür & Lorenz Adrian & Harmen Hawer & Klaus Mayer & Xiaodi Gong & Leonardo Castanedo & Anna Schulten & Nadežda Janina & Marcus Peters & Markus Wirtz & Ulrich , 2022. "Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31712-7
    DOI: 10.1038/s41467-022-31712-7
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    References listed on IDEAS

    as
    1. Ya-Chieh Hsu & Joshua J. Chern & Yi Cai & Mingyao Liu & Kwang-Wook Choi, 2007. "Drosophila TCTP is essential for growth and proliferation through regulation of dRheb GTPase," Nature, Nature, vol. 445(7129), pages 785-788, February.
    2. Yang Zhang & Xuling Zhu & Andrew T. Torelli & Michael Lee & Boris Dzikovski & Rachel M. Koralewski & Eileen Wang & Jack Freed & Carsten Krebs & Steven E. Ealick & Hening Lin, 2010. "Diphthamide biosynthesis requires an organic radical generated by an iron–sulphur enzyme," Nature, Nature, vol. 465(7300), pages 891-896, June.
    3. Eric Linster & Francy L. Forero Ruiz & Pavlina Miklankova & Thomas Ruppert & Johannes Mueller & Laura Armbruster & Xiaodi Gong & Giovanna Serino & Matthias Mann & Rüdiger Hell & Markus Wirtz, 2022. "Cotranslational N-degron masking by acetylation promotes proteome stability in plants," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
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