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A single-cell transcriptomic atlas characterizes the silk-producing organ in the silkworm

Author

Listed:
  • Yan Ma

    (Southwest University)

  • Wenhui Zeng

    (Southwest University)

  • Yongbing Ba

    (Shanghai OE Biotech. Co., Ltd.)

  • Qin Luo

    (Southwest University)

  • Yao Ou

    (Southwest University)

  • Rongpeng Liu

    (Southwest University)

  • Jingwen Ma

    (Southwest University)

  • Yiyun Tang

    (Southwest University)

  • Jie Hu

    (Southwest University)

  • Haomiao Wang

    (Southwest University)

  • Xuan Tang

    (Shanghai OE Biotech. Co., Ltd.)

  • Yuanyuan Mu

    (Southwest University)

  • Qingjun Li

    (Southwest University)

  • Yuqin Chen

    (Southwest University)

  • Yiting Ran

    (Southwest University)

  • Zhonghuai Xiang

    (Southwest University)

  • Hanfu Xu

    (Southwest University)

Abstract

The silk gland of the domesticated silkworm Bombyx mori, is a remarkable organ that produces vast amounts of silk with exceptional properties. Little is known about which silk gland cells execute silk protein synthesis and its precise spatiotemporal control. Here, we use single-cell RNA sequencing to build a comprehensive cell atlas of the silkworm silk gland, consisting of 14,972 high-quality cells representing 10 distinct cell types, in three early developmental stages. We annotate all 10 cell types and determine their distributions in each region of the silk gland. Additionally, we decode the developmental trajectory and gene expression status of silk gland cells. Finally, we discover marker genes involved in the regulation of silk gland development and silk protein synthesis. Altogether, this work reveals the heterogeneity of silkworm silk gland cells and their gene expression dynamics, affording a deeper understanding of silk-producing organs at the single-cell level.

Suggested Citation

  • Yan Ma & Wenhui Zeng & Yongbing Ba & Qin Luo & Yao Ou & Rongpeng Liu & Jingwen Ma & Yiyun Tang & Jie Hu & Haomiao Wang & Xuan Tang & Yuanyuan Mu & Qingjun Li & Yuqin Chen & Yiting Ran & Zhonghuai Xian, 2022. "A single-cell transcriptomic atlas characterizes the silk-producing organ in the silkworm," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31003-1
    DOI: 10.1038/s41467-022-31003-1
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    References listed on IDEAS

    as
    1. Gioele La Manno & Ruslan Soldatov & Amit Zeisel & Emelie Braun & Hannah Hochgerner & Viktor Petukhov & Katja Lidschreiber & Maria E. Kastriti & Peter Lönnerberg & Alessandro Furlan & Jean Fan & Lars E, 2018. "RNA velocity of single cells," Nature, Nature, vol. 560(7719), pages 494-498, August.
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    Cited by:

    1. Wenbo Hu & Anqiang Jia & Sanyuan Ma & Guoqing Zhang & Zhaoyuan Wei & Fang Lu & Yongjiang Luo & Zhisheng Zhang & Jiahe Sun & Tianfang Yang & TingTing Xia & Qinhui Li & Ting Yao & Jiangyu Zheng & Zijie , 2023. "A molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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