IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30606-y.html
   My bibliography  Save this article

Transgenic Anopheles mosquitoes expressing human PAI-1 impair malaria transmission

Author

Listed:
  • Tales V. Pascini

    (National Institutes of Health)

  • Yeong Je Jeong

    (National Institutes of Health)

  • Wei Huang

    (Johns Hopkins Bloomberg School of Public Health)

  • Zarna R. Pala

    (National Institutes of Health)

  • Juliana M. Sá

    (National Institutes of Health)

  • Michael B. Wells

    (Johns Hopkins University School of Medicine
    Idaho College of Osteopathic Medicine)

  • Christopher Kizito

    (Johns Hopkins Bloomberg School of Public Health)

  • Brendan Sweeney

    (National Institutes of Health)

  • Thiago L. Alves e Silva

    (National Institutes of Health)

  • Deborah J. Andrew

    (Johns Hopkins University School of Medicine)

  • Marcelo Jacobs-­Lorena

    (Johns Hopkins Bloomberg School of Public Health)

  • Joel Vega-Rodríguez

    (National Institutes of Health)

Abstract

In mammals, the serine protease plasmin degrades extracellular proteins during blood clot removal, tissue remodeling, and cell migration. The zymogen plasminogen is activated into plasmin by two serine proteases: tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), a process regulated by plasminogen activator inhibitor 1 (PAI-1), a serine protease inhibitor that specifically inhibits tPA and uPA. Plasmodium gametes and sporozoites use tPA and uPA to activate plasminogen and parasite-bound plasmin degrades extracellular matrices, facilitating parasite motility in the mosquito and the mammalian host. Furthermore, inhibition of plasminogen activation by PAI-1 strongly blocks infection in both hosts. To block parasite utilization of plasmin, we engineered Anopheles stephensi transgenic mosquitoes constitutively secreting human PAI-1 (huPAI-1) in the midgut lumen, in the saliva, or both. Mosquitoes expressing huPAI-1 strongly reduced rodent and human Plasmodium parasite transmission to mosquitoes, showing that co-opting plasmin for mosquito infection is a conserved mechanism among Plasmodium species. huPAI-1 expression in saliva induced salivary gland deformation which affects sporozoite invasion and P. berghei transmission to mice, resulting in significant levels of protection from malaria. Targeting the interaction of malaria parasites with the fibrinolytic system using genetically engineered mosquitoes could be developed as an intervention to control malaria transmission.

Suggested Citation

  • Tales V. Pascini & Yeong Je Jeong & Wei Huang & Zarna R. Pala & Juliana M. Sá & Michael B. Wells & Christopher Kizito & Brendan Sweeney & Thiago L. Alves e Silva & Deborah J. Andrew & Marcelo Jacobs-­, 2022. "Transgenic Anopheles mosquitoes expressing human PAI-1 impair malaria transmission," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30606-y
    DOI: 10.1038/s41467-022-30606-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30606-y
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-022-30606-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Junitsu Ito & Anil Ghosh & Luciano A. Moreira & Ernst A. Wimmer & Marcelo Jacobs-Lorena, 2002. "Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite," Nature, Nature, vol. 417(6887), pages 452-455, May.
    2. Olena Riabinina & Darya Task & Elizabeth Marr & Chun-Chieh Lin & Robert Alford & David A. O'Brochta & Christopher J. Potter, 2016. "Organization of olfactory centres in the malaria mosquito Anopheles gambiae," Nature Communications, Nature, vol. 7(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Michelle L. Johnson & Bruce A. Hay & Maciej Maselko, 2024. "Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Pranjul Singh & Shefali Goyal & Smith Gupta & Sanket Garg & Abhinav Tiwari & Varad Rajput & Alexander Shakeel Bates & Arjit Kant Gupta & Nitin Gupta, 2023. "Combinatorial encoding of odors in the mosquito antennal lobe," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    3. Vanessa M. Macias & Johanna R. Ohm & Jason L. Rasgon, 2017. "Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?," IJERPH, MDPI, vol. 14(9), pages 1-30, September.
    4. Rosario Vicidomini & Saumitra Dey Choudhury & Tae Hee Han & Tho Huu Nguyen & Peter Nguyen & Felipe Opazo & Mihaela Serpe, 2024. "Versatile nanobody-based approach to image, track and reconstitute functional Neurexin-1 in vivo," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30606-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.