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Kidney epithelial cells are active mechano-biological fluid pumps

Author

Listed:
  • Mohammad Ikbal Choudhury

    (Johns Hopkins University
    Institute of NanoBioTechnology, Johns Hopkins University)

  • Yizeng Li

    (Johns Hopkins University
    Kennesaw State University)

  • Panagiotis Mistriotis

    (Johns Hopkins University
    Auburn University)

  • Ana Carina N. Vasconcelos

    (Johns Hopkins University
    Institute of NanoBioTechnology, Johns Hopkins University)

  • Eryn E. Dixon

    (Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
    Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Jing Yang

    (Johns Hopkins University
    Institute of NanoBioTechnology, Johns Hopkins University)

  • Morgan Benson

    (Institute of NanoBioTechnology, Johns Hopkins University
    Johns Hopkins University)

  • Debonil Maity

    (Institute of NanoBioTechnology, Johns Hopkins University
    Johns Hopkins University)

  • Rebecca Walker

    (Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Leigha Martin

    (Johns Hopkins University)

  • Fatima Koroma

    (Johns Hopkins University)

  • Feng Qian

    (Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Konstantinos Konstantopoulos

    (Institute of NanoBioTechnology, Johns Hopkins University
    Johns Hopkins University)

  • Owen M. Woodward

    (Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Sean X. Sun

    (Johns Hopkins University
    Institute of NanoBioTechnology, Johns Hopkins University)

Abstract

The role of mechanical forces driving kidney epithelial fluid transport and morphogenesis in kidney diseases is unclear. Here, using a microfluidic platform to recapitulate fluid transport activity of kidney cells, we report that renal epithelial cells can actively generate hydraulic pressure gradients across the epithelium. The fluidic flux declines with increasing hydraulic pressure until a stall pressure, in a manner similar to mechanical fluid pumps. For normal human kidney cells, the fluidic flux is from apical to basal, and the pressure is higher on the basal side. For human Autosomal Dominant Polycystic Kidney Disease cells, the fluidic flux is reversed from basal to apical. Molecular and proteomic studies reveal that renal epithelial cells are sensitive to hydraulic pressure gradients, changing gene expression profiles and spatial arrangements of ion exchangers and the cytoskeleton in different pressure conditions. These results implicate mechanical force and hydraulic pressure as important variables during kidney function and morphological change, and provide insights into pathophysiological mechanisms underlying the development and transduction of hydraulic pressure gradients.

Suggested Citation

  • Mohammad Ikbal Choudhury & Yizeng Li & Panagiotis Mistriotis & Ana Carina N. Vasconcelos & Eryn E. Dixon & Jing Yang & Morgan Benson & Debonil Maity & Rebecca Walker & Leigha Martin & Fatima Koroma & , 2022. "Kidney epithelial cells are active mechano-biological fluid pumps," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29988-w
    DOI: 10.1038/s41467-022-29988-w
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    References listed on IDEAS

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    1. Shalaka Chitale & Wenxuan Wu & Avik Mukherjee & Herbert Lannon & Pooja Suresh & Ishan Nag & Christina M. Ambrosi & Rona S. Gertner & Hendrick Melo & Brendan Powers & Hollin Wilkins & Henry Hinton & Mi, 2023. "A semiconductor 96-microplate platform for electrical-imaging based high-throughput phenotypic screening," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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