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Cyst growth in ADPKD is prevented by pharmacological and genetic inhibition of TMEM16A in vivo

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  • Ines Cabrita

    (Universität Regensburg)

  • Andre Kraus

    (Friedrich-Alexander University Erlangen-Nürnberg)

  • Julia Katharina Scholz

    (Friedrich-Alexander University Erlangen-Nürnberg)

  • Kathrin Skoczynski

    (Friedrich-Alexander University Erlangen-Nürnberg)

  • Rainer Schreiber

    (Universität Regensburg)

  • Karl Kunzelmann

    (Universität Regensburg)

  • Björn Buchholz

    (Friedrich-Alexander University Erlangen-Nürnberg)

Abstract

In autosomal dominant polycystic kidney disease (ADPKD) multiple bilateral renal cysts gradually enlarge, leading to a decline in renal function. Transepithelial chloride secretion through cystic fibrosis transmembrane conductance regulator (CFTR) and TMEM16A (anoctamin 1) are known to drive cyst enlargement. Here we demonstrate that loss of Pkd1 increased expression of TMEM16A and CFTR and Cl− secretion in murine kidneys, with TMEM16A essentially contributing to cyst growth. Upregulated TMEM16A enhanced intracellular Ca2+ signaling and proliferation of Pkd1-deficient renal epithelial cells. In contrast, increase in Ca2+ signaling, cell proliferation and CFTR expression was not observed in Pkd1/Tmem16a double knockout mice. Knockout of Tmem16a or inhibition of TMEM16A in vivo by the FDA-approved drugs niclosamide and benzbromarone, as well as the TMEM16A-specific inhibitor Ani9 largely reduced cyst enlargement and abnormal cyst cell proliferation. The present data establish a therapeutic concept for the treatment of ADPKD.

Suggested Citation

  • Ines Cabrita & Andre Kraus & Julia Katharina Scholz & Kathrin Skoczynski & Rainer Schreiber & Karl Kunzelmann & Björn Buchholz, 2020. "Cyst growth in ADPKD is prevented by pharmacological and genetic inhibition of TMEM16A in vivo," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18104-5
    DOI: 10.1038/s41467-020-18104-5
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    Cited by:

    1. Dohui Kim & Hyeonji Lim & Jaeseung Youn & Tae-Eun Park & Dong Sung Kim, 2024. "Scalable production of uniform and mature organoids in a 3D geometrically-engineered permeable membrane," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Mohammad Ikbal Choudhury & Yizeng Li & Panagiotis Mistriotis & Ana Carina N. Vasconcelos & Eryn E. Dixon & Jing Yang & Morgan Benson & Debonil Maity & Rebecca Walker & Leigha Martin & Fatima Koroma & , 2022. "Kidney epithelial cells are active mechano-biological fluid pumps," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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