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Redesigning regulatory components of quorum-sensing system for diverse metabolic control

Author

Listed:
  • Chang Ge

    (Beijing University of Chemical Technology)

  • Zheng Yu

    (Beijing University of Chemical Technology)

  • Huakang Sheng

    (Beijing University of Chemical Technology)

  • Xiaolin Shen

    (Beijing University of Chemical Technology)

  • Xinxiao Sun

    (Beijing University of Chemical Technology)

  • Yifei Zhang

    (Beijing University of Chemical Technology)

  • Yajun Yan

    (The University of Georgia)

  • Jia Wang

    (Beijing University of Chemical Technology)

  • Qipeng Yuan

    (Beijing University of Chemical Technology)

Abstract

Quorum sensing (QS) is a ubiquitous cell–cell communication mechanism that can be employed to autonomously and dynamically control metabolic fluxes. However, since the functions of genetic components in the circuits are not fully understood, the developed QS circuits are still less sophisticated for regulating multiple sets of genes or operons in metabolic engineering applications. Here, we discover the regulatory roles of a CRP-binding site and the lux box to −10 region within luxR-luxI intergenic sequence in controlling the lux-type QS promoters. By varying the numbers of the CRP-binding site and redesigning the lux box to −10 site sequence, we create a library of QS variants that possess both high dynamic ranges and low leakiness. These circuits are successfully applied to achieve diverse metabolic control in salicylic acid and 4-hydroxycoumarin biosynthetic pathways in Escherichia coli. This work expands the toolbox for dynamic control of multiple metabolic fluxes under complex metabolic background and presents paradigms to engineer metabolic pathways for high-level synthesis of target products.

Suggested Citation

  • Chang Ge & Zheng Yu & Huakang Sheng & Xiaolin Shen & Xinxiao Sun & Yifei Zhang & Yajun Yan & Jia Wang & Qipeng Yuan, 2022. "Redesigning regulatory components of quorum-sensing system for diverse metabolic control," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29933-x
    DOI: 10.1038/s41467-022-29933-x
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    References listed on IDEAS

    as
    1. In Young Hwang & Elvin Koh & Adison Wong & John C. March & William E. Bentley & Yung Seng Lee & Matthew Wook Chang, 2017. "Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    2. Yuheng Lin & Xiaolin Shen & Qipeng Yuan & Yajun Yan, 2013. "Microbial biosynthesis of the anticoagulant precursor 4-hydroxycoumarin," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    3. Yaping Yang & Yuheng Lin & Jian Wang & Yifei Wu & Ruihua Zhang & Mengyin Cheng & Xiaolin Shen & Jia Wang & Zhenya Chen & Chenyi Li & Qipeng Yuan & Yajun Yan, 2018. "Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
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