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Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis

Author

Listed:
  • Yaping Yang

    (The University of Georgia)

  • Yuheng Lin

    (The University of Georgia)

  • Jian Wang

    (The University of Georgia)

  • Yifei Wu

    (Beijing University of Chemical Technology
    Beijing University of Chemical Technology)

  • Ruihua Zhang

    (The University of Georgia)

  • Mengyin Cheng

    (The University of Georgia)

  • Xiaolin Shen

    (Beijing University of Chemical Technology
    Beijing University of Chemical Technology)

  • Jia Wang

    (Beijing University of Chemical Technology
    Beijing University of Chemical Technology)

  • Zhenya Chen

    (Beijing University of Chemical Technology
    Beijing University of Chemical Technology)

  • Chenyi Li

    (The University of Georgia)

  • Qipeng Yuan

    (Beijing University of Chemical Technology
    Beijing University of Chemical Technology)

  • Yajun Yan

    (The University of Georgia)

Abstract

Writing artificial logic and dynamic function into complex cellular background to achieve desired phenotypes or improved outputs calls for the development of new genetic tools as well as their innovative use. In this study, we present a sensor-regulator and RNAi-based bifunctional dynamic control network that can provide simultaneous upregulation and downregulation of cellular metabolism for engineered biosynthesis. The promoter-regulator-mediated upregulation function and its transduced downregulation function through RNAi are systematically verified and characterized. We apply this dynamic control network to regulate the phosphoenolpyruvate metabolic node in Escherichia coli and achieve autonomous distribution of carbon flux between its native metabolism and the engineered muconic acid biosynthetic pathway. This allows muconic acid biosynthesis to reach 1.8 g L−1. This study also suggests the circumstances where dynamic control approaches are likely to take effects.

Suggested Citation

  • Yaping Yang & Yuheng Lin & Jian Wang & Yifei Wu & Ruihua Zhang & Mengyin Cheng & Xiaolin Shen & Jia Wang & Zhenya Chen & Chenyi Li & Qipeng Yuan & Yajun Yan, 2018. "Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05466-0
    DOI: 10.1038/s41467-018-05466-0
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    Cited by:

    1. Xianglai Li & Zhao Zhou & Wenna Li & Yajun Yan & Xiaolin Shen & Jia Wang & Xinxiao Sun & Qipeng Yuan, 2022. "Design of stable and self-regulated microbial consortia for chemical synthesis," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Jian Wang & Yuxi Teng & Ruihua Zhang & Yifei Wu & Lei Lou & Yusong Zou & Michelle Li & Zhong-Ru Xie & Yajun Yan, 2021. "Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    3. Chang Ge & Zheng Yu & Huakang Sheng & Xiaolin Shen & Xinxiao Sun & Yifei Zhang & Yajun Yan & Jia Wang & Qipeng Yuan, 2022. "Redesigning regulatory components of quorum-sensing system for diverse metabolic control," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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