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Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models

Author

Listed:
  • In Young Hwang

    (Yong Loo Lin School of Medicine, National University of Singapore
    NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore)

  • Elvin Koh

    (Yong Loo Lin School of Medicine, National University of Singapore
    NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore)

  • Adison Wong

    (Yong Loo Lin School of Medicine, National University of Singapore
    NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore)

  • John C. March

    (Cornell University)

  • William E. Bentley

    (University of Maryland)

  • Yung Seng Lee

    (NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore
    Yong Loo Lin School of Medicine, National University of Singapore)

  • Matthew Wook Chang

    (Yong Loo Lin School of Medicine, National University of Singapore
    NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore)

Abstract

Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice). These findings support the further development of engineered microorganisms with potential prophylactic and therapeutic activities against gut infections.

Suggested Citation

  • In Young Hwang & Elvin Koh & Adison Wong & John C. March & William E. Bentley & Yung Seng Lee & Matthew Wook Chang, 2017. "Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15028
    DOI: 10.1038/ncomms15028
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    Cited by:

    1. Brian D. Huang & Thomas M. Groseclose & Corey J. Wilson, 2022. "Transcriptional programming in a Bacteroides consortium," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Austin G. Rottinghaus & Aura Ferreiro & Skye R. S. Fishbein & Gautam Dantas & Tae Seok Moon, 2022. "Genetically stable CRISPR-based kill switches for engineered microbes," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    3. Elvin Koh & In Young Hwang & Hui Ling Lee & Ryan De Sotto & Jonathan Wei Jie Lee & Yung Seng Lee & John C. March & Matthew Wook Chang, 2022. "Engineering probiotics to inhibit Clostridioides difficile infection by dynamic regulation of intestinal metabolism," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Betz, Ulrich A.K. & Arora, Loukik & Assal, Reem A. & Azevedo, Hatylas & Baldwin, Jeremy & Becker, Michael S. & Bostock, Stefan & Cheng, Vinton & Egle, Tobias & Ferrari, Nicola & Schneider-Futschik, El, 2023. "Game changers in science and technology - now and beyond," Technological Forecasting and Social Change, Elsevier, vol. 193(C).
    5. Chang Ge & Zheng Yu & Huakang Sheng & Xiaolin Shen & Xinxiao Sun & Yifei Zhang & Yajun Yan & Jia Wang & Qipeng Yuan, 2022. "Redesigning regulatory components of quorum-sensing system for diverse metabolic control," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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