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Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands

Author

Listed:
  • Richard Peter Owen

    (University of Oxford)

  • Michael Joseph White

    (University of Oxford)

  • David Tyler Severson

    (University of Oxford)

  • Barbara Braden

    (University of Oxford)

  • Adam Bailey

    (University of Oxford)

  • Robert Goldin

    (St Mary’s Hospital, Imperial College)

  • Lai Mun Wang

    (Oxford University Hospitals NHS Foundation Trust)

  • Carlos Ruiz-Puig

    (University of Oxford)

  • Nicholas David Maynard

    (Oxford University Hospitals)

  • Angie Green

    (University of Oxford)

  • Paolo Piazza

    (University of Oxford
    Imperial College London)

  • David Buck

    (University of Oxford)

  • Mark Ross Middleton

    (Old Road Campus Research Building, Roosevelt Drive)

  • Chris Paul Ponting

    (MRC IGMM, University of Edinburgh)

  • Benjamin Schuster-Böckler

    (University of Oxford)

  • Xin Lu

    (University of Oxford)

Abstract

Barrett’s oesophagus is a precursor of oesophageal adenocarcinoma. In this common condition, squamous epithelium in the oesophagus is replaced by columnar epithelium in response to acid reflux. Barrett’s oesophagus is highly heterogeneous and its relationships to normal tissues are unclear. Here we investigate the cellular complexity of Barrett’s oesophagus and the upper gastrointestinal tract using RNA-sequencing of single cells from multiple biopsies from six patients with Barrett’s oesophagus and two patients without oesophageal pathology. We find that cell populations in Barrett’s oesophagus, marked by LEFTY1 and OLFM4, exhibit a profound transcriptional overlap with oesophageal submucosal gland cells, but not with gastric or duodenal cells. Additionally, SPINK4 and ITLN1 mark cells that precede morphologically identifiable goblet cells in colon and Barrett’s oesophagus, potentially aiding the identification of metaplasia. Our findings reveal striking transcriptional relationships between normal tissue populations and cells in a premalignant condition, with implications for clinical practice.

Suggested Citation

  • Richard Peter Owen & Michael Joseph White & David Tyler Severson & Barbara Braden & Adam Bailey & Robert Goldin & Lai Mun Wang & Carlos Ruiz-Puig & Nicholas David Maynard & Angie Green & Paolo Piazza , 2018. "Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06796-9
    DOI: 10.1038/s41467-018-06796-9
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    Cited by:

    1. Mohammad Faujul Kabir & Adam L. Karami & Ricardo Cruz-Acuña & Alena Klochkova & Reshu Saxena & Anbin Mu & Mary Grace Murray & Jasmine Cruz & Annie D. Fuller & Margarette H. Clevenger & Kumaraswamy Nai, 2022. "Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Naveen Kumar & Pon Ganish Prakash & Christian Wentland & Shilpa Mary Kurian & Gaurav Jethva & Volker Brinkmann & Hans-Joachim Mollenkopf & Tobias Krammer & Christophe Toussaint & Antoine-Emmanuel Sali, 2024. "Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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