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LC3B is an RNA-binding protein to trigger rapid mRNA degradation during autophagy

Author

Listed:
  • Hyun Jung Hwang

    (Korea University
    Korea University)

  • Hongseok Ha

    (Korea University
    Korea University)

  • Ban Seok Lee

    (Korea University
    Korea University)

  • Bong Heon Kim

    (Korea University)

  • Hyun Kyu Song

    (Korea University)

  • Yoon Ki Kim

    (Korea University
    Korea University)

Abstract

LC3/ATG8 has long been appreciated to play a central role in autophagy, by which a variety of cytoplasmic materials are delivered to lysosomes and eventually degraded. However, information on the molecular functions of LC3 in RNA biology is very limited. Here, we show that LC3B is an RNA-binding protein that directly binds to mRNAs with a preference for a consensus AAUAAA motif corresponding to a polyadenylation sequence. Autophagic activation promotes an association between LC3B and target mRNAs and triggers rapid degradation of target mRNAs in a CCR4-NOT–dependent manner before autolysosome formation. Furthermore, our transcriptome-wide analysis reveals that PRMT1 mRNA, which encodes a negative regulator of autophagy, is one of the major substrates. Rapid degradation of PRMT1 mRNA by LC3B facilitates autophagy. Collectively, we demonstrate that LC3B acts as an RNA-binding protein and an mRNA decay factor necessary for efficient autophagy.

Suggested Citation

  • Hyun Jung Hwang & Hongseok Ha & Ban Seok Lee & Bong Heon Kim & Hyun Kyu Song & Yoon Ki Kim, 2022. "LC3B is an RNA-binding protein to trigger rapid mRNA degradation during autophagy," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29139-1
    DOI: 10.1038/s41467-022-29139-1
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    1. Sukjun Kim & Soyoung Kim & Hee Ryung Chang & Doyeon Kim & Junehee Park & Narae Son & Joori Park & Minhyuk Yoon & Gwangung Chae & Young-Kook Kim & V. Narry Kim & Yoon Ki Kim & Jin-Wu Nam & Chanseok Shi, 2021. "The regulatory impact of RNA-binding proteins on microRNA targeting," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    2. Xiao Wang & Zhike Lu & Adrian Gomez & Gary C. Hon & Yanan Yue & Dali Han & Ye Fu & Marc Parisien & Qing Dai & Guifang Jia & Bing Ren & Tao Pan & Chuan He, 2014. "N6-methyladenosine-dependent regulation of messenger RNA stability," Nature, Nature, vol. 505(7481), pages 117-120, January.
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