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Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

Author

Listed:
  • Angelina Schindele

    (Karlsruhe Institute of Technology)

  • Fabienne Gehrke

    (Karlsruhe Institute of Technology)

  • Carla Schmidt

    (Karlsruhe Institute of Technology)

  • Sarah Röhrig

    (Karlsruhe Institute of Technology)

  • Annika Dorn

    (Karlsruhe Institute of Technology)

  • Holger Puchta

    (Karlsruhe Institute of Technology)

Abstract

CRISPR/Cas has been mainly used for mutagenesis through the induction of double strand breaks (DSBs) within unique protein-coding genes. Using the SaCas9 nuclease to induce multiple DSBs in functional repetitive DNA of Arabidopsis thaliana, we can now show that cell death can be induced in a controlled way. This approach, named CRISPR-Kill, can be used as tool for tissue engineering. By simply exchanging the constitutive promoter of SaCas9 with cell type-specific promoters, it is possible to block organogenesis in Arabidopsis. By AP1-specific expression of CRISPR-Kill, we are able to restore the apetala1 phenotype and to specifically eliminate petals. In addition, by expressing CRISPR-Kill in root-specific pericycle cells, we are able to dramatically reduce the number and the length of lateral roots. In the future, the application of CRISPR-Kill may not only help to control development but could also be used to change the biochemical properties of plants.

Suggested Citation

  • Angelina Schindele & Fabienne Gehrke & Carla Schmidt & Sarah Röhrig & Annika Dorn & Holger Puchta, 2022. "Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29130-w
    DOI: 10.1038/s41467-022-29130-w
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    References listed on IDEAS

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    1. Tonni Grube Andersen & Sadaf Naseer & Robertas Ursache & Brecht Wybouw & Wouter Smet & Bert De Rybel & Joop E. M. Vermeer & Niko Geldner, 2018. "Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells," Nature, Nature, vol. 555(7697), pages 529-533, March.
    2. Tonni Grube Andersen & Sadaf Naseer & Robertas Ursache & Brecht Wybouw & Wouter Smet & Bert Rybel & Joop E. M. Vermeer & Niko Geldner, 2018. "Author Correction: Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells," Nature, Nature, vol. 559(7714), pages 9-9, July.
    3. Carla Schmidt & Paul Fransz & Michelle Rönspies & Steven Dreissig & Jörg Fuchs & Stefan Heckmann & Andreas Houben & Holger Puchta, 2020. "Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
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