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Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

Author

Listed:
  • Carla Schmidt

    (Botanical Institute, Karlsruhe Institute of Technology)

  • Paul Fransz

    (University of Amsterdam)

  • Michelle Rönspies

    (Botanical Institute, Karlsruhe Institute of Technology)

  • Steven Dreissig

    (Martin Luther University Halle Wittenberg)

  • Jörg Fuchs

    (Leibniz Institute of Plant Genetics and Crop Plant Research)

  • Stefan Heckmann

    (Leibniz Institute of Plant Genetics and Crop Plant Research)

  • Andreas Houben

    (Leibniz Institute of Plant Genetics and Crop Plant Research)

  • Holger Puchta

    (Botanical Institute, Karlsruhe Institute of Technology)

Abstract

Chromosomal inversions are recurrent rearrangements that occur between different plant isolates or cultivars. Such inversions may underlie reproductive isolation in evolution and represent a major obstacle for classical breeding as no crossovers can be observed between inverted sequences on homologous chromosomes. The heterochromatic knob (hk4S) on chromosome 4 is the most well-known inversion of Arabidopsis. If a knob carrying accession such as Col-0 is crossed with a knob-less accession such as Ler-1, crossovers cannot be recovered within the inverted region. Our work shows that by egg-cell specific expression of the Cas9 nuclease from Staphylococcus aureus, a targeted reversal of the 1.1 Mb long hk4S-inversion can be achieved. By crossing Col-0 harbouring the rearranged chromosome 4 with Ler-1, meiotic crossovers can be restored into a region with previously no detectable genetic exchange. The strategy of somatic chromosome engineering for breaking genetic linkage has huge potential for application in plant breeding.

Suggested Citation

  • Carla Schmidt & Paul Fransz & Michelle Rönspies & Steven Dreissig & Jörg Fuchs & Stefan Heckmann & Andreas Houben & Holger Puchta, 2020. "Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18277-z
    DOI: 10.1038/s41467-020-18277-z
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    Cited by:

    1. Qichao Lian & Victor Solier & Birgit Walkemeier & Stéphanie Durand & Bruno Huettel & Korbinian Schneeberger & Raphael Mercier, 2022. "The megabase-scale crossover landscape is largely independent of sequence divergence," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Angelina Schindele & Fabienne Gehrke & Carla Schmidt & Sarah Röhrig & Annika Dorn & Holger Puchta, 2022. "Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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