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Deep tissue localization and sensing using optical microcavity probes

Author

Listed:
  • Aljaž Kavčič

    (J. Stefan Institute)

  • Maja Garvas

    (J. Stefan Institute
    CENN Nanocenter)

  • Matevž Marinčič

    (J. Stefan Institute
    University of Ljubljana)

  • Katrin Unger

    (Graz University of Technology, NAWI Graz)

  • Anna Maria Coclite

    (Graz University of Technology, NAWI Graz)

  • Boris Majaron

    (University of Ljubljana
    J. Stefan Institute)

  • Matjaž Humar

    (J. Stefan Institute
    CENN Nanocenter
    University of Ljubljana)

Abstract

Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues, imaging at single-cell resolution beyond the ballistic light transport regime remains very challenging. Here, we show that optical microcavity probes embedded inside cells enable three-dimensional localization and tracking of individual cells over extended time periods, as well as sensing of their environment, at depths well beyond the light transport length. This is achieved by utilizing unique spectral features of the whispering-gallery modes, which are unaffected by tissue scattering, absorption, and autofluorescence. In addition, microcavities can be functionalized for simultaneous sensing of various parameters, such as temperature or pH value, which extends their versatility beyond the capabilities of standard fluorescent labels.

Suggested Citation

  • Aljaž Kavčič & Maja Garvas & Matevž Marinčič & Katrin Unger & Anna Maria Coclite & Boris Majaron & Matjaž Humar, 2022. "Deep tissue localization and sensing using optical microcavity probes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28904-6
    DOI: 10.1038/s41467-022-28904-6
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    References listed on IDEAS

    as
    1. Dragan Maric & Jahandar Jahanipour & Xiaoyang Rebecca Li & Aditi Singh & Aryan Mobiny & Hien Nguyen & Andrea Sedlock & Kedar Grama & Badrinath Roysam, 2021. "Whole-brain tissue mapping toolkit using large-scale highly multiplexed immunofluorescence imaging and deep neural networks," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Alasdair H. Fikouras & Marcel Schubert & Markus Karl & Jothi D. Kumar & Simon J. Powis & Andrea Di Falco & Malte C. Gather, 2018. "Non-obstructive intracellular nanolasers," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    3. Ekaterina I. Galanzha & Robert Weingold & Dmitry A. Nedosekin & Mustafa Sarimollaoglu & Jacqueline Nolan & Walter Harrington & Alexander S. Kuchyanov & Roman G. Parkhomenko & Fumiya Watanabe & Zeid Ni, 2017. "Spaser as a biological probe," Nature Communications, Nature, vol. 8(1), pages 1-7, August.
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